摘要
目的以急性淋巴细胞白血病(ALL)患者骨髓基质细胞(BMSC)构建白血病细胞黏附介导耐药(CAM-DR)模型,从一新的角度探讨 CAM-DR 在白血病细胞耐药中的作用。方法采用人淋巴细胞白血病 Jurkat 细胞株与经^(60)Co 射线照射后的 ALL 骨髓基质细胞贴壁层共培养构建 CAM-DR模型,应用扫描电镜从形态及结构对该模型进行鉴定;四甲基偶氮唑盐比色法(MTF 法)和流式细胞仪分别测定柔红霉素(DNR)对 Jurkat 细胞的细胞毒作用、DNR 在 Jurkat 细胞中的储积浓度及分布。结果扫描电镜结果显示 CAM-DR 模型中 Jurkat 细胞通过伪足黏附于骨髓基质细胞层,或移行到骨髓基质细胞层下,并可见 Jurkat 细胞呈巢状龛于骨髓基质细胞"网眼"中。流式细胞结果显示该模型并末影响 DNR 在 Jurkat 细胞中的蓄积及药物的分布,但显著抑制了 Jurkat 细胞对 DNR 的反应性。结论成功构建了以白血病 BMSC 为基础的 CAM-DR 模型,为进一步研究白血病细胞耐药机制奠定了理论和实验基础。
Objective To construct cell adhesion mediated drug resistance （CAM-DR） model based on Acute lymphocyte leukemia bone marrow stromal cells（BMSC） for further studying drug resistance of leukemia. Methods Firstly, we adhesively cultured Jurkat cell strain of human leukaemia lymphocyte with the matrix cell radiated by ^60Co to construct the model of CAM-DR, then elatuated the model in morph and construction by scanning electron microscope. The ICS0 of DNR on Jurkat cell was examed by MTT and the concentration and distribution of DNR in the cell was detected by flow cytometry. Results When the Jurkat cells were cultured with BMSC for 24 h, we found that Jurkat cells had adhsioned the bone marrow stromal cell layer by parapodium and some of them had niched the mesh constited by the confluence of BMSC. At the point of 48 h, some Jurkat cells had migrated to underlayer of BMSC, and Jurkat cells were niched the mesh of BMSC like nidi. The accumulation and distribution of DNR in the Jurkat cells were not affected in the model, but the reaction of Jurkat cells to DNR were significantly inhibited. Conclusion The model of CAM-DR based on Acute lymphocyte leukemia bone marrow stromal cells was successfully built.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第20期1380-1383,共4页
National Medical Journal of China
基金
国家自然科学基金(39770335)
关键词
白血病
骨髓基质细胞
多药耐药
细胞黏附
细胞黏附介导耐药
Leukemia
Bone Marrow Stromal Cells
Multi-drug resistance
Cell adhesion
Cell adhesion mediated drug resistance
