摘要
OBJECTIVE To examine the influence of tumor osseous metastasis on the patients undergoing autoiogous peripheral blood stem ceil collection. METHODS A total of 36 patients with malignant diseases who received an autoiogous peripheral blood stem ceil transplantation, during a period from April 2004 to June 2006, were chosen. The patients were divided into two groups, i.e. group A were patients with a complication of tumor osseous metastasis, and group B were without metastasis. Both groups were treated with Taxotere 120 mg/m^2 plus granuiocyte colony-stimulating factor （G-CSF） 5 μg/kg/d, for a mobilization regimen. A blood ceil separator was used to collect the mononuciear ceils. The proportion of harvested CD34＋ ceils in the peripheral blood and the collected mononuciear ceils were detected by flow cytometry. The number of CD34＋ ceils was used to determine the difference in the nature of the collections between the two groups. RESULTS After mobilization in groups A and B, the number of the peripheral blood mononuciear ceils （PBMC） was 39.3±14.7% and 41.1±12.4 % and the proportion of CD34＋ ceils was 0.16±0.07% and 0.17±0.10%, respectively. Following administration of the drugs, there was no significant difference between the number of harvested PBMC and CD34＋ cells of the two groups, i.e., 3.47±1.16×10^8/Kg and 2.52±1.43×10^6/Kg in group A and 4.02±1.31×10^8/Kg and 2.73±1.87×10^6/Kg in group B, respectively. CONCLUSION Osseous metastasis, as a single factor, may have no impact on mobilization and harvesting of hematopoietic stem ceils and their engraftment after autotransplantation.
OBJECTIVE To examine the influence of tumor osseous metastasis on the patients undergoing autologous peripheral blood stem cell collection. METHODS A total of 36 patients with malignant diseases who received an autologous peripheral blood stem cel transplantation,during a period from April 2004 to June 2006,were chosen.The patients were divided into two groups,i.e.group A were patients with a complication of tumor osseous metastasis,and group B were without metastasis.Both groups were treated with Taxotere 120 mg/m2 plus granulocyte colony-stimulating factor(G-CSF) 5μg/kg/d,for a mobilization regimen.A blood cell separator was used to col- lect the mononuclear cel s.The proportion of harvested CD34+cel s in the peripheral blood and the collected mononuclear cells were detected by flow cytometry.The number of CD34+cells was used to determine the difference in the nature of the collections between the two groups. RESULTS After mobilization in groupsA and B,the number of the periph- eral blood mononuclear cel s(PBMC)was 39.3±14.7%and 41.1±12.4 %and the proportion of CD34+cells was 0.16±0.07%and 0.17±0.10%, respectively.Folowing administration of the drugs,there was no significant difference between the number of harvested PBMC and CD34+cells of the two groups,i.e.,3.47±1.16×108/Kg and 2.52±1.43×106/Kg in group A and 4.02±1.31×108/Kg and 2.73±1.87×106/Kg in group B,respectively. CONCLUSION Osseous metastasis,as a single factor,may have no impact on mobilization and harvesting of hematopoietic stem cells and their engraftment after autotransplantation.
