摘要
百合鳞叶细胞培养在附加１～２ｍｇ／Ｌ６－ＢＡ的ＭＳ培养基上进行器官型态发生，在附加２ｍｇ／Ｌ２，４－Ｄ的ＭＳ培养基上进行器官发生型形态发生．电镜观察发现：两种不同途径中胚性细胞的超微结构有明显差异．器官型中胚性细胞形态相似，排列整齐规则，核大，叶绿体结构完整，线粒体与淀粉粒在胞质中分布有规律，但在器官发生型中胚性细胞之间形态有差异，排列不整齐，细胞器在胞质中无明显分布规律，但在数量上除淀粉粒外要比器官型丰富．根据观察结果，在细胞学上提出了胚性细胞中细胞器的结构分布预定其形态发生途径的新观点，即结构位置预定假说．
The results of this study indicate that organs such as buds can form from explants in organ type pathway when explants are cultured on MS medium containing 1￣2 mg/L 6 BA,and in organogey type pathway when cultured on MS medium containing 2 mg/L 2,4 D.The embrynoic cells have differences in ultrastructure between two morphogenesis pathways.In ogran type pathway,embryonic cells are similar in shape.They line in good order,and have larger nucleus,complete structure of chloroplast.Starch grains and mitochondiums have obvious patterns of distribution in cells.In organogey type pathway,embryonic cells are different in shape.They do not have obvious patterns of distribution of cell organs,but are more in cell organ numbers except starch grains. On the basis of the results,the authors put forward a new viewpoint that the “structure and location predefine the morphogenesis type”.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
1997年第1期30-35,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
本研究系湖南省科委资助
关键词
百合
细胞
形态发生
超微结构
胚性细胞
lilium brownii cells morphogenesis ultrastuctures / embryonic cells
