摘要
为了研究地方山羊品种的起源与分化,了解其遗传背景,为山羊资源的合理利用提供基础资料,利用PCR产物直接测序法对3个中国黑山羊群体和1个韩国黑山羊群体共39个个体的mtDNAD-环部分序列进行了测定和分析。测定的序列经排列比对后选取441bp分析,发现了55个多态位点,单一多态位点11个,简约信息位点44个,确定了29种单倍型,单倍型多样度为0.984。构建系统发育树将29种单倍型分成了明显的3个分支,角猾羊聚入A分支。同时利用群体间的单倍型的错配分布和遗传距离分析了各群体的亲缘关系。结果表明:4个黑山羊群体遗传多样性丰富,至少拥有3个不同的母系起源,角猾羊是家山羊的一个母系祖先。
In order to investigate the origin and differentiation of indigenous goat populations, clarify the genetic background to provide the sound utilization with basic information, we sequenced and analysed partial sequence of mtDNA D-loop of 39 black goats from 3 Chinese and 1 Korean populations.441 bps sequence was analyzed, 45 polymorphic sites were defined, including 11 singlton polymohic sites and 44 parsimony polymorphic sites, all the 55 sites defined 29 haplotypes, and haplotype diversity is 0.984. Phylogenetic tree defined 3 major lineages, C.aegagrus clustered with lineage A. The relative relationship among populations were also analyzed through pairwise distribution and genetic distance between haplotyoes. The results show that the 4 black goat populations are abundant in genetic diversity, at least have 3 different maternal origins, C.aegagrus is one of the origins of domestic goat.
出处
《中国农学通报》
CSCD
2007年第6期57-60,共4页
Chinese Agricultural Science Bulletin
基金
科技部科技成果转化基金(05EFN213700167)
山东省肉羊良种工程项目(SDLZ05RY0203)
