摘要
为观察儿童支气管哮喘时Ｔ淋巴细胞亚群分布以及Ｔ细胞活化相关因子及受体的表达状况，应用放射免疫分析等技术，对３４例发作期、２５例缓解期支气管哮喘患儿和１５例正常对照的外周血Ｔ淋巴细胞亚群、血浆及淋巴细胞膜表面白细胞介素－２受体（ＩＬ－２Ｒ）和相关细胞因子等水平进行系统检测。结果：（１）发作期患儿外周血Ｔ细胞亚群ＣＤ３，ＣＤ４及ＣＤ４／ＣＤ８值与缓解期患儿及正常对照比较差异无显著意义，但发作期ＣＤ８高于正常对照（Ｐ＜０．０１）和缓解组（Ｐ＜０．０１）；（２）发作期患儿血浆可溶性ＩＬ－２Ｒ、（ｓＩＬ－２Ｒ）、ＩｇＥ水平明显高于缓解期患儿和正常对照（Ｐ＜０．０１）；（３）免疫电镜观察显示，发作期患儿淋巴细胞膜表面ＩＬ－２Ｒ表达高于正常对照（Ｐ＜０．０５）。提示：（１）哮喘患儿外周Ｔ淋巴细胞亚群的分布发生了变化，哮喘发作期Ｔ淋巴细胞处于激活状态；（２）血浆ｓＩＬ－２Ｒ、ＩｇＥ水平与哮喘病情变化密切相关，可作为临床哮喘病情监测的指标。
To investigate the role of T lymphocyte subsets,cytokines and soluble interleukin 2 receptors (sIL 2R)in children′s bronchial asthma,we measured T lymphocyte subsets,sIL 2R,interleu kin 2 receptors on lymphocyte membrane and cytokines in 34 asthmatic children at the stage of attack,25 at remission stage and 15 normal subjects with radioimmunoassy and immunoelectromicroscopy The results showed that:(1) no differences was found in the mean percentages of CD 3 T and CD 4 T,and the ratio of CD 4 T/CD 8 T among the three groups;but apparant increase in CD 8 T subsets in severe asthma was noted( P <0 01);(2)both sIL 2R and IgE levels in cases of asthma at the stage of attack were significantly higher than those in the cases at remission stage and control subjects ( P <0 01);(3)the IL 2R expression of lymphocyte membranes at attack stage of asthma was higher than that of normal subjects ( P <0 05) This study suggested that:(1)in children with asthma T lymphocyte subsets changed and T lymphocytes were activated;(2)sIL 2R and IgE,to certain extent, might reflect the severity of asthma and could be used as an index for surveillance.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1997年第2期84-87,共4页
Chinese Journal of Pediatrics
关键词
哮喘
T细胞
生物因子
白细胞介素-2
儿童
Asthma T lymphocyte subsets Biological factors Receptors,interleukin 2
