摘要
目的:探讨乙型肝炎患者体内乙肝病毒(HBV)标志物与乙肝病毒脱氧核糖核酸(HBV-DNA)的关系及其临床意义。方法:采用酶联免疫吸附试验(ELISA)检测HBV血清标志物,并对212份HBV标志物阳性标本采用荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA。结果:Ⅰ组(HBsAg、HBeAg、抗-HBc三项阳性)、Ⅱ组(HBsAg、抗-HBe、抗-HBc三项阳性)、Ⅲ组(HBsAg、抗-HBc阳性)血清中HBV-DNA阳性率分别为97.17%、47.13%、47.37%;Ⅰ组血清中HBV-DNA阳性率显著高于Ⅱ组与Ⅲ组血清中HBV-DNA阳性率(P<0.01)。结论:患者血清HBV-DNA的阳性率与HBV血清标志物的存在状态相关。检测乙肝病毒,采用FQ-PCR法检测HBV-DNA能更准确、直接地反映体内病毒复制情况。
Objective： To investigate the relationship and clinical significance between serum HBV markers and HBV-DNA in hepatitis B patients. Methods： Serum HBV markers were detected by ELISA and serum HBV-DNA was detected by fluorescence quantitative polymerase chain reaction （FQ-PCR） technology in the 212 patients whose serum HBV markers were positive. Results： The positive rates of serum HBV-DNA were 97. 17%, 47. 13% and 47. 37% in the group Ⅰ , Ⅱ and Ⅲ group respectively. The positive rate of serum HBV-DNA in the group Ⅰ was significantly higher than the one in the group Ⅱ or in the group Ⅲ. Conclusion： The positive rate of serum HBV-DNA was related to the mode of serum HBV markers. The result of serum HBV-DNA that was detected by FQ-PCR can reflect correctly and directly the replication of hepatitis B virus.
出处
《数理医药学杂志》
2007年第3期319-320,共2页
Journal of Mathematical Medicine
关键词
荧光定量聚合酶链反应
乙型肝炎病毒DNA
酶联免疫吸附分析
HBV血清标志物
fluorescence quantitative polymerase chain reaction （FQ-PCR）
hepatitis B virus deoxyribonucleic acid （HBV-DNA）
enzyme linked immunosorbent assay（ELISA）
serum HBV markers
