摘要
目的:探讨新生大鼠卵巢卵母细胞的凋亡是否与FOXO3a(forkhead box group O)转录因子在细胞核内高表达有关。方法:取新生1d、2d、3d、4d雌性SD大鼠卵巢,用免疫组织化学方法检测FOXO3a与其靶分子促凋亡蛋白BIM的表达变化;TUNEL化学染色观察新生大鼠卵巢中卵母细胞凋亡状况;再用免疫组织荧光联合TUNEL荧光检测技术观察FOXO3a和其靶分子BIM表达水平与卵母细胞凋亡的关系。结果:免疫组织化学结果显示,FOXO3a在一些卵母细胞巢内和原始卵泡的卵母细胞核内高表达,其阳性率在出生后1-2d较高,随后逐渐下降。与FOXO3a相似,BIM也是在一些卵母细胞巢内和原始卵泡的卵母细胞中高表达,阳性率变化规律也与FOXO3a一致。凋亡细胞主要见于卵母细胞巢内和原始卵泡的卵母细胞中,其凋亡率变化与FOXO3a阳性率变化一致。免疫组织荧光联合TUNEL荧光检测结果表明,FOXO3a(核内)与BIM高表达的卵母细胞正是凋亡的卵母细胞。结论:FOXO3a转录因子参与新生大鼠卵巢中卵母细胞巢内卵母细胞和原始卵泡卵母细胞的凋亡调控。
Objective： To explore if oocyte opoptosis is associated with the highly-expressed FOXO3a in nuclei of oocytes in neonatal rat ovaries. Methods： Ovaries were collected from postnatal female SD rats on day 1, 2, 3 and 4 after birth, and examined for the expression of FOXO3a and its target protein BIM with irnmunohistochemistry. The terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling （TUNEL） was used to detect oocyte apoptosis. A combination of immunohistofluorescent and TUNEL-fluorescent techniques was performed to reveal the association of FOXO3a and BIM expressions with oocyte apoptosis. Results： The immunohistochemistry revealed that FOXO3a was highly expressed in the nuclei of some oocytes in nests and oocytes of primordial follicles. BIM was also highly expressed in those oocytes as FOXO3a did. TUNEL showed that the apoptotic oocytes were mainly in oocyte nests and some primordial follicles. The combination of immunohistofluorescent and TUNEL-fluorescent techniques demonstrated that the FOXO3a- and BIM-highly-expressed oocytes were exactly the apoptotic oocytes. Conclusion： FOXO3a is involved in the regulation of oocytes apoptosis in neonatal rat ovaries.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2007年第5期314-319,共6页
Reproduction and Contraception
基金
广东省自然科学基金资助项目
项目编号06033513
