摘要
目的:研究桑色素(morin)对小鼠T淋巴细胞活化、增殖和细胞周期的影响。方法:以刀豆蛋白A(ConA)刺激培养的淋巴结来源的小鼠淋巴细胞,再以不同终浓度的morin与T细胞共培养,利用流式细胞术(FCM),检测早期T细胞活化的标志CD69分子的表达,以羧基荧光素双醋酸盐琥珀酰脂(CFDA-SE)染色检测T细胞的增殖;以碘化丙锭(PI)染色分析T细胞的细胞周期。结果:小鼠T细胞培养6 h后,未经ConA刺激的对照组中CD69+T的细胞比率为(2.97±0.12)%,经ConA刺激的CD69+T细胞的比率明显增高,达到(72.52±0.66)%,与对照组相比差别明显(P<0.01)。终浓度为25、50、100μmol/L的morin均下调CD69+T细胞的比率,其中,100μmol/L的morin抑制作用最强,为(48.95±0.81)%,与对照组比较具有统计学意义(P<0.01)。CFDA-SE染色分析显示,ConA组培养48 h和72 h的T细胞的增殖指数(PI)分别为(1.58±0.04)和(1.95±0.02),各浓度的morin对ConA刺激的T细胞增殖,具有明显地抑制作用,以100μmol/L的morin抑制作用最明显。培养48 h的ConA组T细胞的PI为(1.02±0.02)、培养72 h的ConA组T细胞的PI为(1.03±0.01),与相应时间的对照组比较,均有统计学意义(P<0.01)。PI染色后流式细胞术分析的结果表明,ConA组处于S期的T细胞的比率为(27.05±0.39)%,显著高于对照组的比率(5.10±0.07)%。morin组中S期的细胞比率较高。结论:Morin可显著抑制ConA刺激的T细胞活化及增殖;其对增殖的抑制作用主要表现为S期的细胞的阻滞。
AIM： To discover the effects of morin on the activation, proliferation and cell-cycle of routine T lymphocytes in vitro. METHODS： Murine lymph node-derived T lymphocytes were separated and stimulated with concanavalin A （ConA） and different experimental groups were set by co-cultured with morin of different final concentration. Flow cytometry （FCM） was used to detect the activation, proliferation [ carboxylfluorescein diacetate, succinimide ester （CFDA-SE） staining] and cell-cycle [ propidium iodide （PI） staining] of T cells. RESULTS： After 6 h time of culture in vitro, the rate of CD69＋ T cells in control group was （2.97 ±0. 12 ）%, while it was significant higher in ConA group[ （72.52 ± 0.66）% （ P 〈 0.01 ） ]. Morin could downregulate this rate at final concentration being 25, 50 and 100μmol/L, with a peak at 100 pmol/L morin[ （48.95 ±0.81 ）% （P〈0.01）]. CFDA-SE staining showed that at 48 h and 72 h, the proliferation indexes （P/） of T cells in ConA group were （ 1.58 ± 0.04） and （ 1.95± 0.02）, respectively. Morin could significantly decrease the PI value at all experimental concentration, with the peak effect at 100μmol/L morin, which the P/for 48 h was （1.02±0.02） and （1.03 ±0.01） for72 h （P〈0.01）. FCM analysis of PI staining implied that the percentage of S phase cells in ConA group was （27.05 ± 0.39）%, significantly higher than that in control group （5.10±0. 07）%; and the 25 and 50 iJmoVL morin groups showed higher S phase cell rates. CONCLUSION： Morin can significantly inhibit ConA stimulated activation and proliferation of murine T lymphocytes, in which the S phase lagging may serve as one of the major mechanisms.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2007年第3期197-200,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30230350)
广东省自然科学基金项目(5300413)
关键词
桑色素
T细胞活化
增殖
细胞周期
modn
T cells activation
proliferation
cell cycle
