摘要
目的:了解医疗站物表分离的金黄色葡萄球菌毒力、耐药性及消毒剂抗性,找出优势克隆株,为控制由金黄色葡萄球菌引起的交叉感染提供依据。方法:采集宁波市江北区农村医疗站物表,分离金黄色葡萄球菌,进行药物敏感试验,产肠毒素A-E试验以及消毒剂抗性试验。结果:从382份物表中共分离出18株金黄色葡萄球菌。分别来自医护人员手3株(3/56),诊疗台面5株(5/86),配药台面1株(1/40),器械表面2株(2/105),输液椅、架4株(4/54),病床、床头柜等2株(2/31),工作服1株(1/9)。耐药性试验结果显示菌株对21种药物耐药,最多耐药种类达18种,出现13种耐药谱,菌株存在多重耐药性。消毒剂抗性试验结果发现医疗站物表分离的金黄色葡萄球菌对医院常用消毒剂:新洁尔灭、过氧乙酸、75%酒精、安尔碘、过氧化氢、戊二醛,作用2 min,最低杀灭率为63.33%;作用5 min,最低杀灭率为96.33%;作用10 min,杀灭率为100%。结论:农村医疗站物表中存在着较严重的金黄色葡萄球菌的潜在危害,同时也存在着交叉感染的危机。物表的金黄色葡萄球菌多重耐药性,提示合理使用治疗药物的重要性。金黄色葡萄球菌对医疗机构常用的消毒剂的敏感性,说明农村医疗站只要正确掌握消毒技术,合理运用消毒剂,就能避免或减少院内交叉感染。
Objective：To investigate the virulence, drug resistance and disinfectant resistance of Staphylococcus aureus isolated from surface of substance in medical stations. Methods：The virulence, drug resistance and disinfectant resistance of Staphylococcus aureus isolated from surface of substance in medical stations in Ningbo rural area were determined. Results：Eighteen strains of Staphylococcus aureus were isolated from 382 samples. Of the 18 strains,3 were isolated from the hands of doctors and nurses（3/ 56） ,5 from the consulting counter（ 5/86 ）, 1 from drug dispensing table （1/40）, 2 from medical instruments （2/105）,4 from transfusion chair and framework（4/54） ,2 from bedside box（2/31 ） and 1 from clothes of medical professionals（1/9）. They were resistant to 21 kinds of antibiotics. The most resistant variety was 18 kinds and there were 13 kinds of resistant spectrums. The strains had multi - drug resistance. Meanwhile, they had different resistant rates to various disinfectants. Conclusion： Antibiotics should be reasonably used in medical stations in rural area. Meanwhile, reasonable use of disinfectants is important for reducing cross infection.
出处
《中国卫生检验杂志》
CAS
2007年第5期899-901,共3页
Chinese Journal of Health Laboratory Technology
基金
宁波市江北区科技项目(2006B0301)
关键词
农村医疗站
金黄色葡萄球菌
毒力
耐药性
消毒剂抗性
Rural medical station
Staphylococcus aureus
Toxicity
Resistance
Disinfectant resistance
