摘要
背景与目的云南个旧为矿工职业性肺癌的高发区,既往本课题小组以永生化人支气管上皮细胞(i mmortalized human bronchial epithelial cells,BEAS-2B)为靶细胞,以采自井下作业面的个旧矿粉为染毒物,成功建立了个旧矿粉体外诱导的恶性转化细胞模型。本研究旨在探讨生存素(survivin)和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在个旧矿粉诱导BEAS-2B恶性转化为肺癌细胞过程中发挥的作用。方法采用免疫荧光细胞化学和免疫组织化学技术检测survivin和PCNA在BEAS-2B及其恶性转化肺癌细胞中的表达。结果①免疫荧光细胞化学:survivin蛋白在BEAS-2B中无表达,在其恶性转化肺癌细胞中表达阳性;PCNA蛋白在BEAS-2B中低表达,在其恶性转化肺癌细胞中高表达。②免疫组织化学:survivin蛋白在BEAS-2B中无表达,显著低于在其恶性转化肺癌细胞中的表达率(85%,17/20)(P<0.001);PCNA蛋白在BEAS-2B中的标记指数(labelling index,LI)显著低于其恶性转化肺癌细胞(P<0.001);survivin表达阳性的恶性转化肺癌细胞中PCNA的LI明显高于survivin表达阴性者(P<0.001)。结论①survivin在恶性转化肺癌细胞中表达上调,提示其可能在个旧矿粉诱导BEAS-2B恶性转化为肺癌细胞的过程中发挥重要作用,为揭示个旧矿工肺癌的致癌机制提供依据,其有可能作为个旧矿工肺癌的检测和治疗的新靶点。②PCNA在恶性转化肺癌细胞中表达上调,提示细胞增殖过程可能在该恶性转化过程中发挥重要作用。③sur-vivin可能通过PCNA的介导促进细胞的增殖,二者可能在该恶性转化过程中发挥协同作用。
Background and objective Gejiu in Yunnan Province is a region where the incidence of lung cancer is high among the miners of tin mine. Our previous research team successfully induced the malignant transformation of immortalized human bronchial epithelial cells （BEAS-2B） by Gejiu mineral powder in vitro. The objective of this study is to explore the role of survivin and proliferating cell nuclear antigen （PCNA） in the pathway of the malignant transformation of BEAS-2B induced by Gejiu mineral powder. Methods Protein expression of survivn and PCNA in BEAS-2B cells and its malignant transformation cells was respectively evaluated by immunofluorescence cytochemlstry staining technique and immunohistochemistry. Results ①The expression of survivin protein was negative in BEAS-2B cells and was positive in its malignant transformation cells by immunofluorescence cytochemistry staining techniques. And the protein level of PCNA was low in BE- AS-2B cells and high in its malignant transformation cells. ②The positive expression of survivin protein in BE- AS-2B cells （0/20） was significantly lower than that in its malignant transformation cells （85%, 17/20） by immunohlstochemistry （P〈0. 001 ）. And the labelling index （LI） of PCNA in BEAS-2B cells was significantly lower than that in its malignant transformation cells （P〈0. 001）. LI of PCNA in the malignant transformation cells of BEAS-2B with positive expression of survivin was significantly higher than that with negative expression of survivin （P〈0. 001）. Conclusion ①The up-regulation expression of survivin in malignant transformation cells of BEAS-2B suggests that survivin may play an important role in the pathway of malignant transformation in BEAS-2B cells induced by Gejiu mineral powder. It may give a proof for revealing the carcinogenesis of lung cancer in Gejiu miner. Survivin may he identified as a novel potential diagnostic and therapeutic target of lung cancer in Gejiu miner. ②The up-regulation expression of PCNA in malignant transformation cells of BEAS-2B suggests that cell proliferation may play an important role in the pathway of malignant transformation. ③Survivin may promote cell proliferation mediated by PCNA. Survivn and PCNA may play synergetic roles in the process of malignant transformation in BEAS-2B cells induced by Gejiu mineral powder.
出处
《中国肺癌杂志》
CAS
2007年第2期83-87,共5页
Chinese Journal of Lung Cancer
基金
国家自然科学基金(30560053)
高等学校博士学科点专项科研基金(20050678004)资助~~
关键词
生存素
增殖细胞核抗原
肺肿瘤
个旧矿粉
永生化人支气管上皮细胞
Survivin Proliferating cell nuclear antigen Lung neoplasms Gejiu mineral powder Immortalized human bronchial epithelial cells
