摘要
目的了解肿节风注射液抗肿瘤及与阿霉素联合用药的效果。方法采用四氮唑盐法(MTT法)测定药物的体外抑制作用,用IC50评测直接抗肿瘤效果,用金氏公式进行联合用药分析。结果肿节风注射液能抑制人肝癌细胞Bel7402、人结肠癌细胞HCT-8的增殖,其IC50分别为33.13,52.39mg/mL。3.125,6.25,12.5mg/mL的肿节风注射液与阿霉素联合对HCT-8细胞体外抑制呈相加作用;25,50mg/mL的肿节风注射液与阿霉素联合对HCT-8细胞体外抑制呈协同作用。结论肿节风注射液对Bel7402、HCT-8细胞有一定的抑制肿瘤生长的作用,它与阿霉素联合应用对HCT-8细胞可产生相加或增强的协同抑制效果,尤其是高浓度的肿节风注射液与阿霉素的协同作用更为显著。
Objective To study the anti -tumor effect of Zhongjiefeng Injection in vitro and its combination with adriamyein. Methods MTT assay was adopted to determine the in - vitro anti - tumor effect, IC50 was used to measure the direct anti - tumor effect, and Jing＇s formula was applied to analyze the combination of the drugs. Results Zhongjiefeng Injection can inhibit the proliferation of Bel 7402 （human hepatoma cells）, HCT - 8 （ human colon cancer cells）, and the IC50 was 33. 13 mg/mL for Bel 7402 and 52. 39 mg/mL for HCT - 8 respectively. Zhongjiefeng Injection at the concentrations of 3. 125, 6.25, 12.5 mg/mL showed an effieaney potentiation action with doxorubiein on the inhibition of HCT - 8 cells in vitro, and 25, 50 mg/mL showed a synergie effect on HCT - 8 . Conclusion Zhongjiefeng Injection has a certain in - vitro inhibitory effect on the growth of Bel 7402 and HCT - 8, its combination with doxorubiein in vitro can produce synergie effeets（a simple combined or enhanced effects） to HCT- 8 cells, especially high concentrations of Zhongjiefeng Injection with doxorubiein. It is suggested that Zhong,jiefeng Injection in the doubled dosage may have a better synergistic effect with doxorubiein in clinical treatment of colon cancer.
出处
《中药新药与临床药理》
CAS
CSCD
2007年第3期200-202,216,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
