摘要
目的观察人原发性肝细胞肝癌患者肝脏Kupffer细胞(KCs)的分布并探讨其意义。方法采用免疫组织化学方法,通过CD68定位KCs,运用ImageTool3.0软件进行细胞计数,了解KCs在正常人肝组织、不同分化程度肝癌组织、癌旁肝组织和远癌肝组织的分布,并在电子显微镜下观察KCs的超微结构改变。结果KCs呈卵圆形、星形或纺锤形,在肝癌组织、癌旁肝组织、远癌肝组织和正常肝组织中分布数量分别为(21.6±7.8)、(68.8±9.1)、(62.0±1.9)和(36.2±4.1)(P<0.01),癌组织分化程度越低,其内KCs数量越少(P<0.05)。细胞超微结构观察结果显示,肝癌患者癌旁肝组织肝血窦中KCs数量明显增多,可见中性粒细胞、淋巴细胞和嗜酸性粒细胞浸润,细胞质吞噬体和溶酶体增多。结论原发性肝癌患者肝组织内KCs明显激活,但KCs在癌组织内分布数量明显减少,而且癌组织分化程度越低其内KCs数量越少,而在癌旁肝组织和远癌肝组织内较癌组织和正常肝组织显著增多,提示KCs可能通过复杂的途径参与对肝癌的防御作用。
Objective To observe the distribution of KCs in liver in human primary hepatocellular carcinoma and investigate the significance. Methods KCs were confirmed by immunochemistry of CD68. Image Tool 3.0 software and JEM 2000 electron microscope were employed to define the distribution of KCs in normal human liver tissues, cancer tissues, tissues around and apart from cancer, as well as the number of KCs in different differentiated cancer tissues. Results The KCs revealed orbicular-ovate, astroid or fusiform, the number was （21.6 ±7.8）, （68.8 ±9.1）, （62.0 ±1.9） and （56.2 ±4.1） respectively in liver cancer tissues, tissues around cancer, tissues apart from cancer, and normal human liver tissues （P 〈 0.01 ）, and the number decreased with the degradation of differentiation degree （P 〈 0.05 ）. Electron microscopy found infiltrations of neutrophils, lymphocytes and eosinophile granulocytes, increased number of KCs in hepatic sinusoid in the tissues around cancer, and increased phagosomes and lysosomes in KCs. Conclusion KCs are confirmed greatly activated in liver cancer tissues. The number of KCs declines in liver cancer tissues but increases in tissues around cancer and apart from cancer compared to normal human tissues. This indicates that KCs may participate in the defense to liver cancer by intricate means.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第11期1076-1078,共3页
Journal of Third Military Medical University
