摘要
将含有αA干扰素基因的DNA片段接上化学合成的EcoR I-Hind III转换接头,然后插入酵母分泌型表达载体YFD 18中,构建成质粒YFD 66和YFD66-0.转化酵母菌BJl991后,得到转化子BJ1991/YFD66和BJl991/YFD66-0.干扰素活性测定表明,每升BJ1991/YFD66培养液含有αA干扰素10~6U,而BJ1991/YFD66-0则不产生干扰素,YFD66为αA干扰素表达质粒。
A chemically synthesized EcoR I-Hind III adaptor was added to the 3′ end ofIFNαA gene fragment The latter was then inserted at Hind III site of the yeastsecretory expression vector YFD18. The plasmid resulted was named as YFD 66. Bytransforming YFD66 into the yeast BJ 1991, the engineered yeast BJ1991/YFD 66could express IFNαA in an amount of about 10~8 U/L. In PAGE followed by thesilver staining, an extra band appeared when compared with BJ 1991 without theplasmid. The major component of impurities of secretory protein in the mediahad a molecular weight higher than 25 kD.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1990年第4期368-373,共6页
Journal of Fudan University：Natural Science
关键词
酵母
干扰素
分泌
基因表达
酿酒
interferon, secretion, yeast
gene expression.
