摘要
背景与目的:研究表明,KAI1基因对多种恶性肿瘤细胞具有转移抑制作用。本研究旨在通过探讨KAI1基因对具有高转移潜能的人肝癌细胞株MHCC97-H粘附作用的影响,揭示其转移抑制的分子生物学机制。方法:将载有KAI1基因的质粒转染入具有高转移潜能的MHCC97-H细胞,观察细胞的生长状态,运用ELISA、Western blot等方法测定细胞产生的可溶性细胞间粘附因子-1(sICAM-1)和E-钙粘连蛋白的变化,并通过集落形成实验、细胞粘附实验判定细胞的粘附特点。结果:转染KAI1基因的MHCC97-H细胞形态与转染前无明显差别,但胞浆内黑染颗粒增加;同时细胞分泌的sICAM-1以及细胞内E-钙粘连蛋白较转染前减少,在传代后第4天分别减少24.28%和26.02%。3h及4h的细胞粘附率分别下降11.34%和24.00%,克隆形成率则没有明显变化。结论:KAI1基因改变了MHCC97-H细胞的生长方式和细胞增殖情况,使细胞分泌的sICAM-1以及细胞内E-钙粘连蛋白的量明显减少,使细胞粘附率降低。
BACKGROUND ＆ OBJECTIVE： Metastasis suppressor gene KAI1 can inhibit the metastasis potential of many malignant tumors. This study was to explore the effect of KAI1 gene on the adhesion of hepatocellular carcinoma cell line MHCC97-H with high metastatic potential, and to find the possible mechanism. METHODS：The plasmid containing KAI1 gene was transfected into MHCC97-H cells. The growth status of the cells was observed. The changes of adhesion factors, soluble intercellular adhesive molecule-1 （sICAM-1） and E-cadherin, in the cells were detected by ELASA and Western blot. The character of cell adhesion was determined through plate colony formation test and cell adhesion test. RESULTS： The morphology of MHCC97-H cells did not change markedly after transfection of KAI1 gene, but black granules in cytoplasm were increased. Four days after transfection, the expression of sICAM-1 and E-cadherin were decreased by 24.28% and 26.02%. The adhesion rate was decreased by 11.34% at 3 h after transfection, and by 24.00% at 4 h after transfection, but the cloning efficiency did not change much. CONCLUSION： KAI1 gene could affect the growth pattern and proliferation of MHCC97-H cells, suppress sICAM-1 secretion and E-cadherin production, and inhibit adhesion of MHCC97-H cells.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2007年第5期498-503,共6页
Chinese Journal of Cancer
