摘要
目的探讨5-氮-2'脱氧胞苷(5-Aza-CdR)对人卵巢癌3AO及SKOV3细胞增殖凋亡及抑癌基因RUNX3表达的影响。方法以浓度为0.5、5、50μmol/L的5-Aza-CdR处理人卵巢癌细胞株3AO及SKOV3 3d,常规培养5 d后采用四唑盐(MTT)比色法观察细胞的生长活性,以半定量RT-PCR法检测抑癌基因RUNX3mRNA的表达,并用流式细胞仪检测细胞凋亡率。结果5-Aza-CdR能明显抑制肿瘤细胞生长,随5-Aza-CdR浓度增加,细胞生长速率下降;药物处理后RUNX3 mRNA的表达明显高于处理前(P<0.05),且存在明显剂量依赖性。5-Aza-CdR处理后3AO及SKOV3细胞凋亡率均高于处理前(P均<0.05),细胞凋亡率与5-Aza-CdR剂量均呈正相关。结论5-Aza-CdR能使RUNX3基因去甲基化,增强其抑癌功能。
[Objective] To investigate the eeffect of 5-Aza-2＇-deoxyeytidine （5-Aza-Cdr） on the proliferation and apoptosis of ovarian cells and the expression of tumor suppressor gene RUNX3. [Methods] Human ovarian cancer cell line 3AO and SKOV3 were treated with 5-Aza-CdR （0. 5, 5, 50μmol/L respectively）, a specific demethylating agent for 3 d,and then cultured in RPMI 1640 medium for 5 d. The growth of 3AO and SKOV3 cells was observed by MTT assay before and after 5-Aza-CdR treatment respectively. The expression of RUNX3 mRNA was observed by semi-quantitative reverse transeription-polymerase chain reaction （RT-PCR）. The apoptosis rate of 3AO and SKOV3 cells was analyzed by flow eytometry.[Results] 3AO and SKOV3 cells treated with 5-Aza-CdR displayed a slow growth in comparison with the control cells,and the growth rate decreased with the increasing of 5-Aza-CdR concentration. RUNX3 mRNA expression was increased in a concentration dependent manaer after 5-Aza-CdR treatment （P〈0. 05）. The apoptosis rates after treatment were higher than those before treatment in 3AO and SKOV3 （P〈0. 05）, and had a positive correlation with 5-Aza-CdR dose. [Conclusions] 5-Aza-2＇-deoxyeytidine can inhibit the proliferation and induce the apoptosis of 3AO and SKOV3 cells by inducing demethylation and thereby enhancing RUNX3 gene.
出处
《山东医药》
CAS
北大核心
2007年第11期21-23,共3页
Shandong Medical Journal
