摘要
以海芋的根状茎为外植体,探索了不同的生长调节剂配比对愈伤组织诱导、增殖与分化的影响。结果表明:含TDZ0.5mg/L的MS培养基上愈伤诱导率最高,达到53.3%;4mg/LBA+0.1mg/LNAA生长调节剂浓度有利于愈伤组织的增殖;附加1mg/LBA+0.05mg/LNAA的培养基上芽分化率最高;下表面切除0.1～0.2cm有利于愈伤组织增殖与分化。不定芽在无生长调节剂的1/2MS培养基中都能生根。
Effects of different growth regulators combination on callus inducement, proliferation and differentiation were studied with rhizomes of Alocasia rnacrorrhiza as explants. Results showed that callus inducement rate was maximum and attained 53.3% in MS culture medium containing 0. 5mg/L TDZ; It was advantageous for callus to proliferate with 4mg/L BA＋0. 1 mg/L NAA; Explants possessed the highest differentiation rate in culture medium with lmg/L BA＋ 0. 05mg/L NAA. Callus excised 0. 1-0. 2cm thick in below surface was prone to proliferate and differentiat. When shoots were inserted into culture medium of 1/2MS,all of them produced roots.
出处
《北方园艺》
CAS
北大核心
2007年第5期212-213,共2页
Northern Horticulture
关键词
海芋
离体培养
愈伤增殖
分化
Alocasia rnacrorrhiza
In vitro culture
Callus proliferation
Differentiation
