摘要
通过对斜纹夜蛾核多角体病毒(SpltNPV)在其不同龄期宿主体内增殖情况的研究,探索保幼激素类似物(JHA)methoprene促进SpltNPV在其宿主体内增殖的途径,为今后广泛利用methoprene进行高效率、低成本增殖昆虫杆状病毒杀虫剂提供理论依据和方法.运用正交组合的方法筛选3种主要因子(病毒感染浓度、methoprene用量和methoprene施用时间)对提高病毒增殖产量进行最佳组合实验.实验确立了以1×107PIBs/mL感染6龄刚蜕皮的斜纹夜蛾幼虫,并于感染第1天滴加10μL methoprene的最佳组合方法,病毒总产量和平均病毒产量分别可达1 856.0×108PIBs和62.2×108PIBs.并测定了methoprene处理对SpltNPV病毒LC50的影响.经methoprene处理后,病毒的LC50由1.578×107PIBs/mL增加至9.151×107PIBs/mL.
In order to find the way to promote production of Spodoptera litura nucleopolyhedrovirus （SpltNPV） with the treatment of juvenilehormone analogues （JHA）, the research of multiplication of SpltNPV in different instar larvae of Spodoptera litura was studied, which will be helpful to production and application of virus insecticide with low cost and high efficient. The optimal combination of infection concentration of SpltNPV, the dose and the employed time of methoprene was studied by orthogonal design. The results showed that the highest virus yield could be obtained by treating the larvae which had been infected virus at the concentration of 1 × 10^7 PIBs/mL not more than 12 h with 10 μL methoprene per larva. The total virus yield and average amount of polyhedra per larva were 1 856.0 × 10^8 PIBs and 62.2 × 10^8 PIBs respectively. Finally, the effect of methoprene on median lethal concentration （LC50） of SpltNPV was researched. The result showed that LC50 increased from 1.578 × 10^7 PIBs/mL to 9. 151 × 10^7 PIBs/mL treated with methoprene.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第3期308-312,316,共6页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金资助项目(30170628)
广东省科技攻关资助项目(2003C20509)
肇庆市科技项目(203301)
