摘要
目的研究小鼠注射HIV gag DNA疫苗后的抗原特异性细胞免疫应答。方法C57BL/6小鼠以初免/加强的策略经肌肉注射HIV gag DNA疫苗,一周后获取其脾与肺的单个细胞,体外经Gag抗原多肽刺激后,采用ELISA法检测细胞培养上清中IFN-γ的水平,ELISPOT法检测IFN-γ分泌细胞的频率,流式细胞仪分析特异性T细胞的亚群。结果经Gag多肽刺激后,加强免疫组IFN-γ产生的总体水平和分泌细胞频率均高于对照组及初次免疫组。HIV gag DNA疫苗可同时诱导产生Gag-特异性CD4+和CD8+T细胞。结论HIV gag DNA疫苗免疫小鼠后可诱导抗原特异性效应性T细胞应答。
Objective To elucidate specific T-cell response in mice immunized with HIV gag DNA vaccine. Methods C57BL/6 mice were intramuscularly primed and/or boosted with HIV gag DNA vaccine. Single cells prepared from spleens and lungs were incubated with a pool of HIV Gag peptides in vitro one week post immunization. The levels of Gag-specific IFN-γ in the culture supematants were detected by ELISA; the frequency of Gag-specific IFN-γ-producing cells was assessed by ELISPOT; the specific T-cell sub-populations were analyzed by FACS.Results After stimulation with Gag peptides, the levels of IFN-γ in culture supematats from spleens and lungs of mice boosted with HIV gag DNA vaccine were significantly higher than those of control and primed mice （ both P 〈 0.05）. Similarly the frequency of IFN-γ-producing cells was higher in mice boosted with HIV gag DNA vaccine than those in control and primed mice （both P 〈0.05）. Further analysis indicated that both CD4^＋ and CD8^＋ T-cell subsets mediated the immune responses. Conclusion Mice immunized with HIV gag DNA vaccine can induce specific effector T-cell response.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第3期283-286,共4页
Immunological Journal
基金
广东省自然科学基金重点项目(04105349)
广州市科技局创新药物专项(2004Z3E4031)资助
