摘要
目的:探讨人巨细胞病毒(HCMV)感染后病毒基因表达活性与细胞病变的关系。方法:不同滴度HCMV感染传代培养的人胚肺成纤维细胞,以3个浓度接种HCMV AD169毒株稀释液:10-5(A组)、10-3(B组)、10-1(C组),建立HCMV梯度感染细胞模型,分别应用FQ-PCR法测定感染细胞内HCMV基因拷贝数、RT-PCR法测定MCP mRNA转录水平,同时光镜观察致细胞病变作用(CPE)、电镜检查细胞超微结构改变。结果:HCMV感染后,HCMV DNA在病毒滴度最低的A组细胞内负荷量最低(P<0.05,P<0.01),复制速度也最慢;仅在病毒滴度较高的B、C组内检出MCP mRNA,水平持续升高。A组未发现细胞病变,B、C组细胞检测到随感染时间延长而加重的CPE及超微结构改变。结论:HCMV感染细胞内病毒基因高负荷及MCP mRNA转录与细胞病变进展密切相关。
AIM： To explore the relationship between human cytomegalovirus（HCMV） gene activity and cytopathogenesis in HCMV infection. METHODS： HCMV echelin - infected cell model was set up in vitro by coincubating passage cultured HEL and HCMV with different titers （group A： 10^-5; group B： 10^-3; and group C： 10^-1）. FQ-PCR was performed to evaluate the number of HCMV DNA copies, MCP mRNA was measured by RT - PCR, Meanwhile the development of cytopathologic effects （CPE） was observed utpder microscope and ultrastructural changes determined by TEM. RESULTS： Compared with group B and C in high HCMV titer, group A with low viral titer showed low HCMV DNA load （P 〈0. 05, P 〈0. 01 ）, while in group B and C, major capsid protein（MCP） mRNA was detected. The two latter groups carried developing CPE and ultrastructural impairment with duration of HCMV infection, but that wasn＇t detected in group A. CONCLUSION： High level of HCMV DNA load and successful transcription of MCP mRNA are associated with developing cytopathogenesis in HCMV infcetion.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第5期1013-1016,共4页
Chinese Journal of Pathophysiology
