摘要
目的:探讨乙酰肝素酶(heparanase,HPA)及缺氧诱导因子-1α(hypoxia inducible factors-1α,HIF-1α)在食管鳞癌中的表达及其与食管鳞癌浸润转移的关系。方法:应用原位杂交技术检测70例食管鳞癌组织和23例切缘正常组织中HPA mRNA表达,同时应用免疫组化法检测HIF-1α蛋白在全部标本中的表达。结果:癌组织中HPA mRNA和HIF-1α的阳性表达率分别为65.7%(46/70)和55.7%(39/70),显著高于切缘正常组织(P<0.05);HPA mRNA和HIF-1α的表达与食管鳞癌组织浸润深度、淋巴结转移有关(P<0.05);在食管鳞癌组织中HPA mRNA的表达与HIF-1α的表达关系密切(Kappa=0.615,P<0.05)。结论:HPA和HIF-1α阳性表达与食管鳞癌的生长及浸润转移有关,两者在食管鳞癌的发生、发展过程中可能存在着一定的协同作用;检测HPA与HIF-1α表达有助于食管鳞癌生物学性能的判断。
Objective： To investigate the relationship of expression of HPA and HIF-1α in human esophageal squamous cell carcinoma with its invasion and metastasis. Methods：The expression of HPA mRNA and HIF-1α protein in 23 normal tissue specimens of incised margin and 70 esophageal squamous cell carcinoma specimens were examined by in situ hybridization assay and immunohistochemical stain respectively. Results： HPA mRNA and HIF-1α protein were positive in 65.7% and 55.7% of all 70 cancer tissue specimens, respectively,which were significantly higher than those in incised margin normal tissues（P 〈0.05）. The high expression of HPA mRNA and HIF-1α protein in the esophageal squamous cell carcinoma was significantly correlated with fibromembranous infiltration and lymph node metastasis（ P 〈0.05 ）. There was a close correlation between the expression of HPA mRNA and HIF-1α protein in the esophageal squamous carcinoma tissues （Kappa = 0. 615 ,P 〈 0.05 ）. Conclusion： The high expression of HPA mRNA and HIF-1α protein is correlated with carcinogenesis progression,invasion and metastasis of esophageal squamous cell carcinoma. There is a synergic action between them in the carcinogenesis and development of esophageal carcinoma. The determination of HPA and HIF-1α will be useful for predicting tumor biological behavior.
出处
《军事医学科学院院刊》
CSCD
北大核心
2007年第2期133-136,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
食管肿瘤
乙酰肝素酶
缺氧诱导因子-1Α
肿瘤浸润
原位杂交
esophageal neoplasms
heparanase
hypoxia inducible factors-1α
neoplasm invasiveness
in situ hybridization
