摘要
在已克隆的盐藻(Dunaliella salina)3-磷酸甘油脱氢酶GPD基因的基础上,利用实时荧光定量PCR的方法,研究了经高盐、厌氧、磷酸盐以及氧化等不同外界胁迫条件下该基因的表达情况,并同时监测了盐藻细胞甘油合成的动态变化.对比酿酒酵母的GPD基因,发现D.salina GPD基因受高渗诱导,同时D.salina细胞内可能存在多个形式的GPD基因.
Basing on the cloned Glycerol 3-phosphate dehydrogenase gene of Dunaliella salina （DsGPD）＇, its expression under salt, oxidative, phosphate and anaerobiosis stress wore researched with fluorescent real time PCR. At the same time, the change of total glycerol synthesis of the cells was monitored under the same stress treatments. Contrast with GPD gene of Saccharomyces cerevisiae ,osmotic stress significantly induces DsGPD expression and it was suggestd that there may be other types of DsGPD.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期433-438,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(30500287)
关键词
盐生杜氏藻
3-磷酸甘油脱氢酶
荧光定量PCR
甘油合成
Dunaliella salina, glycerol 3-phosphate dehydrogenase, fluorescent real time PCR, glycerol synthesis
