摘要
目的:建立同时测定复方坎地沙坦酯片中坎地沙坦酯和氢氯噻嗪含量的HPLC-UV法。方法:采用Phenomenex C_(18)(250mm×4.6mm,5μm)色谱柱,流动相为乙腈-0.1mol·L^(-1)磷酸二氢钠溶液(加0.1%三乙胺,磷酸调pH5.0)(65∶35),流速1.0mL·min^(-1),检测波长262nm,柱温35℃。结果:坎地沙坦酯和氢氯噻嗪分别在1.6～32.0μg·mL^(-1)(r=0.9998)和1.2～23.8μg·mL^(-1)(r=0.9999)浓度范围内线性关系良好;平均回收率(n=9)分别为102.1%和100.7%。结论:本文方法准确、快速,适合于复方坎地沙坦酯片的含量测定。
Objective：An HPLC - UV method for simultaneous determination of candesartan cilexetil and hydrochlorothiazide in compound candesartan cilexetil tablets was established. Method： A Phenomenex C18 column（250 mm×4.6mm,5μm） was used with the mobile phase of acetonitrile -0. 1 mol · L^-1 sodium dihydrogen phosphate solution （added 0. 1% triethylamine,adjusted pH S. 0 with phosphoric acid） （65： 35） at the detection wavelength of 262 nm. The flow rate was 1.0 mL · min^-1 and the column temperature was 35 ℃. Results： The calibration curves of candesartan cilexetil and hydrocldorothiazide were linear in the ranges of 1.6 - 32. 0 μg · mL^- 1 （ r = 0. 9998） and 1.2 - 23.8 μg · mL^- 1 （ r = 0. 9999）, respectively. The average recoveries （ n = 9 ） were 102. 1% and 100. 7%, respectively. Condusion： This method is accurate, quick and suitable for content determination of compound candesartan cilexeti] tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第4期566-568,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
复方坎地沙坦酯片
坎地沙坦酯
氢氯噻嗪
高效液相色谱
同时测定
compound candesartan cilexetil tablets
candesartan cilexetil
hydrocldorothiazide
HPLC
simultaneous determination
