摘要
目的探讨依托咪酯对人急性髓性细胞白血病细胞（HL-60细胞）的毒性作用,及依托咪酯预处理对此毒性作用的影响。方法实验分两部分,第一部分为细胞毒性实验,HL-60细胞随机分为6组,对照组不用依托咪酯处理,其他5组分别加入50、100、250、500、1 000μmol/L依托咪酯,每组分别作用4、8、12、24、48 h,进行下述指标检测。第二部分为预处理实验,HL-60细胞随机分为3组,对照组不用依托咪酯处理,依托咪酯组加入500μmol/L依托眯酯作用24 h,预处理组先加入1μmol/L依托咪酯作用1 h,洗脱后在培养基中孵育4 h,再加入500μmol/L依托咪酯作用24 h。采用MTT实验方法检测细胞活力,采用AnnexinⅤ-PI流式细胞仪检测细胞凋亡。结果依托咪酯可抑制HL-60细胞活力,诱导细胞凋亡,呈浓度和时间依赖性。1μmol/L依托咪酯预处理1 h,4 h后对500μmol/L依托咪酯作用24 h诱发的细胞凋亡具有抑制作用（P＜0．05）。结论依托咪酯通过诱发细胞凋亡,对HL-60细胞的功能产生了抑制作用,1μmol/L依托咪酯刺激1 h预处理可减轻药物自身引起的这种抑制作用。
Objective To investigate the effects of etomidate on HL-60 ceils and the protective effect of preconditioning with small dose etomidate.Methods Human promyelocytic leukemia HL-60 ceils were purchased from Shanghai life science institute and cultured in RPMI-1640 culture medium at 37℃ in 5% CO2 incubator for 24 h. The concentration was 1.5 × 10^5 ceils/ml. The experiment was performed in 2 parts. In part I the ceils were exposed to 50, 100,250, 500 and 1 000μmol/L etomidate respectively and incubated for 4, 8, 12, 24 and 48 h. Ceil viability was measured using MTT assay and apoptosis by flow cytometry using Annexin Ⅴ and propidium iodide staining. In part Ⅱ the ceils were exposed to 1 μmol/L etomidate for 1 h and were allowed to recover for 4 h after etomidate washout, then etomidate 500μmol/L was added and the cells were incubated for 24 h. Ceil viability and apoptosis were assessed as in part Ⅰ. Results Etomidate inhibited viability of HL-60 cells and induced apoptosis in a dose and time dependent fashion. Preconditioning with 1μmol/L etomidate for 1 h attenuated the apoptosis induced by etomidate 500μmol/L. Conclusion Etomidate can inhibit ceil viability and induce apoptosis in a dose and time dependent manner. Preconditioning with small dose etomidate has protective effect.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2007年第3期236-239,共4页
Chinese Journal of Anesthesiology
基金
辽宁省教育厅高等学校科学技术研究项目（05L107）
