摘要
目的：直径１．０～１．４ｍｍ静脉经二步冷冻法保存（第一步－６０℃，第二步－１９６℃）与异体移植，观察维持时限对二步法静脉冷冻保存的影响。方法：家兔６０只，体重２．０～２．５ｋｇ，切取股静脉段（２０～２５ｍｍ），分成６组，每组１０只；对照组为自体静脉移植，余５组在－６０℃中按维持时限５ｍｉｎ、１５ｍｉｎ、３０ｍｉｎ、６０ｍｉｎ、１２０ｍｉｎ分组。用慢降温０．５～１．０℃／ｍｉｎ，快复温后作异体静脉移植。术后经光镜、电镜、血管造影、手术探查等对移植血管的组织学及通畅率检测，采用四格表确切概率法检验。结果：６０ｍｉｎ组内膜保存最好，远期（１２周）通畅率８９．９％与对照组差异无显著意义（Ｐ＞０．０５）；其余各组静脉内膜损伤严重，通畅率与对照组差异显著（Ｐ＜０．０５）。结论：（１）静脉冷冻保存维持时限有一定选择性；（２）６０ｍｉｎ组为最佳维持时限；（３）为临床应用保存异体小静脉修复小血管缺损提供依据。
Objective:Veins(Φ1.0～1.4 mm) were cryopreserved in a two step procedure and transplanted allogenously in order to study the role of holding time in venous cryopreservation.Methods:Femoral veins taken from rabbits,were preserved in two step procedure:holding first at -60℃ for various times (5,15,30,60,120 min) and then shifted into -196℃ in liquid nitrogen for 48 hours.After thawing,part of these cryopreserved veins was used for light and electron microscopic examination and part for allograft with fresh autograft as control,and angiography was performed 1 wk after operation.Results:Veins were shown to be best preserved in the holding time of 60 min.The patency rate was 89.9% in 12 wk after operation with no significant difference from that of the fresh autograft( P ≥0.50),whereas,the veins cryopreserved in other holding times,the damage was severe.The patency rate differed greatly from that of the control( P <0.05).Conclusion:These results indicated that the holding time play an important role in vein cryopreservation,the optimal holding time was 60 min.
出处
《蚌埠医学院学报》
CAS
1997年第1期7-8,共2页
Journal of Bengbu Medical College
关键词
二步冷冻法
冷冻保存
维持时限
静脉移植
vein
a two step freezing procedure
cryopreservation
holding time
