摘要
用气相色谱-质谱联用（GC-MS）方法对超临界CO2萃取出的省沽油种子油中角鲨烯和维生素E进行分析。省沽油种子油的萃取采用超临界CO2萃取技术，压力25MPa，温度50℃，时间2h；种子油用GC-MS方法分析，以正三十二烷为内标，用内标标准曲线法同时测定省沽油种子油中角鲨烯和维生素E的含量。结果表明：超临界CO2萃取种子油的收率为28．83％；所建立的定量分析方法平均回收率在96．0％～99．0％之间，相对标准偏差小于2．8％，线性相关系数大于0．999，方法检测限在2．0～2．5mg／L之间；省沽油种子油中含角鲨烯4.65％维堆安E1.58％．
A method for the analysis of squalene and vitamin E in supercritical CO2 extracts from Staphylea bumalda DC. seed by gas chromatography-mass spectrometry （GC-MS） method was developed. The seed oil was extracted by supercfitical CO2 fluid. The extraction condition was that the extraction pressure,temperature and time were 25 MPa,50 ℃ and 2 h. The seed oil was analyzed by GC-MS method. The contents of squalene and vitamin E in seed oil were simultaneously determined by internal standard curve method using n-dotriacontane as internal standard. The results showed that the extraction ratio of seed oil was 28.83%. The average added standard recovery of the method developed by this paper was in the range of 96.0% - 99.0%, the relative standard deviations （RSD）were less than 2.8%, the correlation coefficients were more than 0.999, and the detection limits were in the range of 2.0 ～ 2.5 mg/L. The contents of squalene and vitamin E in Staphylea bumalda DC. seed oil determined by this paper were 4. 65% and 1.58%, and they are much higher than that in common edible oils.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第2期256-258,289,共4页
Natural Product Research and Development
基金
河南省科技攻关项目(424070106)
关键词
超临界CO2萃取
气相色谱-质谱
省沽油种子
角鲨烯
维生素E
supercritical CO2 extraction
gas chromatography-mass spectrometry
Staphylea bumalda DC. seed
squalene
vitamin E
