摘要
目的探讨KCNJ11基因单核苷酸多态性E23K与2型糖尿病(T2DM)遗传易感性的关系。方法从2型糖尿病患者外周血有核细胞中提取基因组DNA,运用聚合酶链式反应扩增KCNJ11基因包含E23K多态性的DNA片断。将其扩增产物进行电泳分析,在紫外分析仪下观察结果并与正常人对照。结果2型糖尿病患者KCNJ11基因E23K的K等位基因频率(73.5%)显著高于对照组(6.7%)。KK基因型频率(72%)也显著高于对照组(2%)。K等位基因和KK基因型在两组中差异具有统计学意义(P<0.01)。结论提示KCNJ11基因单核苷酸多态性KK直接或间接与2型糖尿病遗传易感性相关。
Objective To investigate the impact of KCNJ11 gene-combined single nucleotide polymorphism （SNP） variation on type 2 diabetes（T2DM）.Method Genomic DNA were extracted from the hemocyte of 100 Chinese residents with T2DM in Anhui.Polymerase chain reactions were used to amplify E23K in the KCNJ11 gene. Then the products were analyzed with electrophoresis.Different outcomes can be observed between normal and T2DM groups by ultraviolet rays.Results The frequency of K allele of the KCNJ11 in type 2 diabetes patients was significantly increased as compared with that in the control subjects（73.5% vs 6.7%,P〈0.01）,also was the geno- typic frequency for KK（72% vs 2%,P〈0.01）.The frequency of K allele and KK genotype showed significant difference between these two groups（P〈0.01）.Conclusion KCNJ11 gene combined KK may directly or indirectly contribute to the genetic susceptility of type 2 diabetes.
出处
《中国血液流变学杂志》
CAS
2007年第1期46-48,共3页
Chinese Journal of Hemorheology
