摘要
为了筛选开发新的抗生素,从海水、海泥以及刺鲳、鳗鱼、黄鱼、安康鱼、鲽鱼、马头鱼的鳃和胃肠道中分离得到195株海洋细菌,以金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、黑曲霉和啤酒酵母作为指示菌,用滤纸片法定性测定它们的抗菌活性,筛选到抗菌活性物质产生菌22株。对其中6个抗菌活性较强的菌株进行16S rDNA序列分析,结果显示,它们分别是克氏菌属的Kocuria rhizophila、葡萄球菌属的Staphylococcus saprophyticus、芽孢杆菌属的Bacillus sphaericus和Bacillus thuringiensis、冷杆菌属的Psychrobacter faecalis和变形菌属的Proteus penneri。系统发育分析表明这6个菌株归于3个类群:1个为高(G+C)%的革兰阳性菌;3个为低(G+C)%的革兰阳性菌;另2个为γ-变形菌。
To explore new antibiotics, seawater and marine sediment, as well as the gills, stomachs or intestines of different kinds of marine fish （psenopsis anomala, muraenesox cinereus, pseudosciaena polyactis, Lophius litulon, Cleisthenes nerensteini and Hippocampus sp.） were collected from east China sea and the adherent bacteria were screened. Total 195 marine bacteria strains were isolated from these samples according to the colony morphology and sampling sources, of which 22 strains showed antimicrobial abilities against staphylococcus aureus, escherichia coli, bacillus subtilis or aspergillus niger in the disk paper tests and six strains exhibited relatively high antimicrobial abilities. 16S rDNA sequences analyses showed these 6 strains are closest match to Kocuria rhizophila, Staphylococcus saprophyticus, Bacillus sphaericus, Bacillus thuringiensis, Psychrobacter faecalis and proteus penneri respectively. Phylogenetic analysis indicated that 1 strain belongs to high （G＋C） % Gram positive bacteria, 3strains belong to low （G＋C） % gram positive bacteria and the other 2 are γ-proteobacteria.
出处
《农机化研究》
北大核心
2007年第5期34-38,共5页
Journal of Agricultural Mechanization Research
基金
国家自然科学基金资助项目(30371703)
