摘要
以尖叶石竹茎段为外植体,从不同分裂素及其它激素配合使用等方面,初步建立了尖叶石竹离体培养再生技术体系。结果表明:愈伤诱导培养基为MS+1.0mg/L 2,4-D+ 0.5mg/L6-BA,分化培养基为MS+0.2mg/L6-BA+4mg/L KT+0.02 mg/L IAA,生根培养基为1/2MS+1.0mg/LIBA+0.01mg/L NAA。为尖叶石竹的遗传转化奠定了基础。
The regeneration system from stem of Dianthus spiculifolius was established by using different eytoldnins combining with other phytohormones. The results indicated that the optional medium for inducing callus was MS＋1. 0mg/L 2,4-D＋0. 5mg/L 6--BA, the optional medium for differentiation was MS＋0. 2mg/L 6-BA＋4mg/L KT＋0. 02 mg/L IAA. and 1/2MS＋1. 0mg/L IBA＋0. 01mg/L NAA for the rooting of adventitious buds. The result are suitable for screening gene-transformed Dianthus spiculifolius plants.
出处
《北方园艺》
CAS
北大核心
2007年第4期210-212,共3页
Northern Horticulture
关键词
尖叶石竹
茎段
组织培养
Dianthus spiculifolius
Stem
Tissue Culture
