摘要
本文叙述了以甲基丙烯酸β羟乙酯为载体,尼龙网为基材,利用二次辐射法(辐射接枝、辐射包埋)制备了固定化葡萄糖氧化酶膜。测定了酶膜的一些性质,制得的酶膜保留活力高、反应通透性好,配以氧电极和CY-4型测氧仪,用于临床诊断血糖测定,准确性、重复性以及使用寿命均能满足医院临床检验要求。
The paper deals with the preparation of immobilized glucose oxidasemembrane by using two steps irradiation (irradiation gratfting, irradiation entrapping).Some properties of membrane were discussed. The immobilized glucose oxidase me-mbrane with oxygen electrode and oxygen analyser can be satisfied with the clinicanalysis for the determinaticn of serum glucose.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1990年第3期168-172,共5页
Journal of Radiation Research and Radiation Processing
关键词
辐射接枝
固定化
葡萄糖氧化酶
Immobilized enzyme membrane
Irradiation grafting
Irradiation entrapping
Nylon net
HEMA
Clinic analysis
