摘要
目的 研究不动杆菌属在NC21板上耐药表型及比率.方法 对临床分离的275株不动杆菌属耐药表型进行分类.结果 β-内酰胺类抗菌药物耐药表型Ⅰ～Ⅴ分别为67.3%、1.8%、1.8%、19.6%和0.7%,8.8%未能分类;庆大霉素、阿米卡星和妥布霉素均敏感的菌株占74.2%,庆大霉素耐药表型7.3%,庆大霉素-阿米卡星耐药表型1.1%,庆大霉素、阿米卡星、妥布霉素耐药表型10.9%,未能分类6.5%;Ⅳ型和庆大霉素、阿米卡星、妥布霉素耐药表型同时存在菌株占8.7%,均对亚胺培南敏感,氨苄西林/舒巴坦对50.0%菌株敏感.结论 表型分类有利于合理使用抗菌药物和对多药耐药菌株监测,亚胺培南、氨苄西林/舒巴坦有较高的抗菌活性,氨曲南不适合不动杆菌属感染的治疗.
OBJECTIVE To investigate the antibiotic resistant phenotyping of Acinetobacter spp on NC21 panel. METHODS A total of 275 strains were isolated from clinical specimens. Acinetobacter were identified and antimicrobial susceptibility test performed. Phenotyping was according to David Livermore. Resistance phenotyping analysis included 13 antibiotics. RESULTS The susceptible results of 13 antimicrobial agents in 275 strains of Acinetobacter spp showed imipenem, amikacin, ampicillin-sulbactam, ciprofloxacin, tobramycin, ceftazidime, cefepime, gentamicin and trimethoprim had the greatest susceptibility （97.8%, 88.4%, 86.6%, 82.2%, 81.9%, 9. 6%, 76.8% ,73.8% and 73.6%, respectively）. Beta-lactam agent-resistant phenotypes Ⅰ to Ⅴ （wild type, penicillinase, cephalosporinase, penicillinase＋cephalosporinase, multi-drug resistance mechanism） and nontyping were 67.3%, 1.8%, 1.8%,19.6%, 0.7% and 8.8%, respectively. 74.2% of 275 strains were susceptible to gentamicin, amikacin and tobramycin. Aminoglycosides resistant phenotype （gentamicin-resistant, gentamicin-amikacin-resistant, gentamicin-amikacin-tobramycin-resistant） and non%yping were 7.3%, 1.1%, 10.9% and 6. 5%, respectively. 8. 7% were the penicillinase-cephalosporinase and gentamicin-amikacintobramycin-resistant phenotyps, those strains were all susceptible to imipenem and half to ampicillin-sulbactam. CONCLUSIONS Resistance phenotyping system should be useful in epidemiological studies of Acinetobacter and avoiding unnecessary antibiotics treatment, ampicillin-sulbactam as imipenem shows high antibacterial activities, aztreonam has a lower antimicrobial susceptibility than other 12 antibiotics.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第4期379-381,共3页
Chinese Journal of Nosocomiology
关键词
不动杆菌属
耐药
表型
Acinetobacter
Resistance
Phenotyping
