摘要
目的建立肠黏膜上皮细胞模型和测定嗜酸乳杆菌的黏附能力。方法将嗜酸乳杆菌用胃蛋白酶和HCl-H2O低pH以及反复冻融、灭活等方法处理后测定其黏附能力。结果成功地建立了鸡胚肠黏膜上皮细胞模型;经胃蛋白酶和HCl-H2O低pH以及灭活处理后,嗜酸乳杆菌的黏附能力和对照组差异有显著性。反复冻融后的嗜酸乳杆菌黏附能力与对照组差异无显著性。结论胃蛋白酶和HCl-H2O pH2.0会使嗜酸乳杆菌黏附肠黏膜上皮细胞能力下降,热灭活可使嗜酸乳杆菌的黏附能力提高,反复冻融对嗜酸乳杆菌的黏附能力无明显影响。
Objective To set up the model of intestinal epithelial cells and test the adhering capacity of Lactobacillus acidophilus. Method Pepsin, HC1-H2O pH 2.0, freeze-thaw cycle, inactivation etc. were used to test the adhering capacity of LactobaciUus acidophilus. Results The model of intestinal epithehal cells of chick embryo was set up successfully; After treated by pepsin, HC1-H2O pH 2.0 and inactivation, the adhering capacity of Lactobacillus acidophilus was significantly lower than that of HH control group ; The adhering capacity of Lactobacillus acidophilus treated by freeze-thaw cycle didnt change obviously compared to the HH control group. Conclusions Pepsin and HC1-H2O pH 2.0 can make the adhering capacity of Lactobacillus acidophilus decreased; Inactivation by heating can make the adhering capacity of Lactobacillus acidophilus improved; Freeze-thaw cycle cant influence the adhering capacity of Lactobacillus acidophilus obviously.
出处
《中国微生态学杂志》
CAS
CSCD
2007年第2期145-147,149,共4页
Chinese Journal of Microecology
基金
博士后基金项目
关键词
嗜酸乳杆菌
肠黏膜上皮细胞
黏附能力
Lactobacillus acidophilus
Intestinal epithehal cells
Adhering capacity
