摘要
目的探索信号途径ERK→AP-1在双歧杆菌的完整肽聚糖(WPG)激活巨噬细胞中的作用。方法以WPG刺激SD大鼠腹腔巨噬细胞,采用凝胶电泳迁移分析技术检测巨噬细胞AP-1的活性。结果WPG刺激组巨噬细胞AP-1的活性明显高于对照组(P<0.01);巨噬细胞经ERK抑制剂PD98059预孵后,其AP-1的活性显著低于WPG刺激组(P<0.01)。结论双歧杆菌的WPG可通过ERK→AP-1这一信号途径来活化巨噬细胞。
Objective To explore the role of whole peptidoglycan（WPG） of Bifidobacteria to activate macrophages in view of signal pathway ERK→AP-1. Method AP-1 activity of macrophages was detected by using electrophoretic mobility shift assay,after WPG stimulated peritoneal macrophages of SD mice. Results AP-1 activity of macrophages in WPG stimulating group was significantly higher than that in control group（ P 〈 0.01 ）. After the macrophages were incubated by ERK inhibitor PD98059, its AP-1 activity was obviously lower than WPG stimulating group. Conclusion WPG of Bifidobacteria could activate macrophages by regulating the signal pathway of ERK→AP-1.
出处
《中国微生态学杂志》
CAS
CSCD
2007年第2期129-131,共3页
Chinese Journal of Microecology
基金
广东省自然基金资助课题(编号:0400696105009077)
关键词
双歧杆菌
完整肽聚糖
巨噬细胞
激活蛋白1
Bifidobacterium； Whole peptidoglycan
Macrophages
Active protein 1
