摘要
目的:了解辽宁省汉坦病毒的分子特征,建立肾综合征出血热(HFRS)病毒基因库。方法:用RT-PCR方法对间接免疫荧光阳性鼠肺标本进行目的基因的扩增并测序,与标准汉坦病毒(HV)株一起进行同源性分析及系统发生树分析。结果:23株扩增产物与L99 HV同源性在78.5%～99.6%之间,属汉城型(SEO)型HV,除[01]Shenyang-Yuhong/5/2005/sewer-r,[02]Shenyang-Yuhong/4/2005/sewer-r,[03]Shenyang-Yuhong/7/2005/sewer-r外,同源性均在94%以上。获得的23株HV序列在系统发生树上的遗传距离较近,同源性也均在94.7%以上,基本属于同一亚型。各株与LR1 HV相应序列进行比较同源性仅在26.7%～43.7%之间。结论:辽宁省是以SEO型HV为主的SEO和汉滩(HTN)混合型疫区,且SEO型汉坦病毒变异较小,稳定性较高。
Objective ：To understand the molecular characteristics of Hantaviruses（HV） and establish the Gene Bank in Liaoning Province. Methods：The lung tissues positive to HV antigens by immunofluorescent assay were amplified by RTPCR with HV specific primers and the products were sequenced. With the known Hantaviruses, 23 PCR products were analyzed in homology and phylogenetics. Results：The homology of the 23 samples was 78.5% -99.6% compared with L99 HV. They were seoul （SEO） - type HV. Except [ 01 ] Shenyang - Yuhong/5/2005/sewer - r and [ 02 ] Shenyang - Yuhong/4/2005/sewer - r and [ 03 ] Shenyang - Yuhong/7/2005/sewer - r, the homology was more than 94% in the rest 20 samples compared with L99 HV. The relationship in 23 PCR products was close in the phylogenetics. The homology was nearly more than 94. 7% ,belonging to the same subtype. However,the homology was only 26. 7% -43.7% compared with LR1 HV. Conclusion：Liaoning province is a complex area with both SEO and HTN HV. The SEO is the main type and the viruses are highly conserved.
出处
《中国卫生检验杂志》
CAS
2007年第4期626-628,共3页
Chinese Journal of Health Laboratory Technology
