摘要
目的:应用酶联免疫法分别检测丙肝病毒核心抗原(HCV-cAg)和抗体(HCV-Ab),了解丙肝病毒核心抗原检测的意义。方法:采用军事医学科学院基础医学研究所研发并由湖南景达基因公司推出的商业化的HCV游离核心抗原试剂盒,对来自入院前或手术前筛查的180例临床样本和24例丙肝或疑似丙肝患者的血清样本进行HCV-cAg和HCV-Ab检测,阳性者用反转录多聚酶链反应(RT-RNA)证实。结果:180例筛查样本HCV-Ab均为阴性,HCV-cAg阳性2例,其中RT-RNA阳性1例;24例HCV-Ab阳性的样本检出HCV-cAg阳性12例,RT-RNA阳性14例。HCV-cAg与RT-RNA符合率为85.71%(12/14)。结论:HCV核心抗原检出时间早于抗体,HCV-cAg检测试剂盒可作为HCV抗体检测的补充试剂,尤其对术前HCV筛查的患者联合应用更具有临床价值。
Objection：To investigate the significance of detection of core antigen to hepatitis c virus by using the internal regent EIA and Anti - HCV. Methods： The enzyme immunoassay kits for HCV - eAg developed by academy of military medical science and Hunan Jingda Gene Co. Ltd. were used in this study. The 180 clinical samples of negative and 24 of positive with anti - HCV were tested with HCV -eAg EIA kits and results were compared with HCVRNA tests. Results：In 180 clinical samples of negative and 24 of positive,positive rates were 1.1% （2/180） and 50% （ 12/24）, the HCV - RNA were 0. 55% （1/180） and 58. 33% （ 14/24）, respectively. Compared with HCV - eAg and RT - RNA,the coincidental rate was 85.71% （12/14）. Condusion：HCV core antigen can be detected in patients with hepatitis prior to antibody production. HCV core antigen EIA may be the additional detection of anti - HCV with certain clinical value.
出处
《中国卫生检验杂志》
CAS
2007年第4期624-625,共2页
Chinese Journal of Health Laboratory Technology
