摘要
目的:对分离自环境与临床患者的6株MRSA通过多种方法进行基因分型。方法:应用多重PCR方法检测该6株金葡菌是否MRSA,然后通过SCCmec typing,MLST,spaA typing,coa typing,AP-PCR,RAPD和ERIC-PCR等多种方法进行基因分型。结果:6株金葡菌均为MRSA,均携带Ⅲ型SCCmec,序列型为ST239,spaA型为GKAOMQ,coa型为HI-JKL,AP-PCR,RAPD和ERIC-PCR的指纹图谱均显示其带型一致,来源于同一克隆系,病原上具有高度同源性。结论:MRSA可能通过环境与患者之间传播而引起感染,并且如果该克隆的MRSA携带上毒素基因,则可能爆发非常严重和危险的院内感染。
Objective： To determine genotypes of 6 environmental and clinical acquired - MRSA strains using several methods. Methods： Six S. aureus strains were subjected to multiplex - PCR for detection of MRSA, then genotyping for all strains was performed using SCCmec typing,MLST,spaA typing,coa typing,AP - PCR,RAPD and ERIC - PCR. Results：The 6 S. aureus strains were ST239 - MRSA -Ⅲ, its spaA type was GKAOMQ and coa type was HIJKL. According to the fingerprinting of AP - PCR,RAPD and ERIC -PCR, 6 MRSA strains exhibited the same patterns, indicating that they were originated from the same clone and were phylogenetieally related. Conclusion： Nosocomial infection between patients and environment could be mediated by MRSA. Moreover, a severe and dangerous MRSA outbreak could occur if a virulent strain is present
出处
《中国卫生检验杂志》
CAS
2007年第4期612-615,共4页
Chinese Journal of Health Laboratory Technology
基金
广东省自然科学基金项目(04020050)
广州市科技计划项目(2004J1-C0161)
