摘要
通过程序性死亡（ＰＣＤ）的几个重要调控蛋白Ｐ５３、ｃ－ｍｙｃ及ｂｃｌ－２在人正常骨髓高度纯化的ＣＤ３４＋造血细胞上的表达水平，了解正常生理状态下，造血细胞是否也存在着ＰＣＤ及其相关的调节机制。方法根据阳性选择策略建立了高效免疫磁性分离系统对人正常骨髓ＣＤ３４＋造血细胞进行了富集，随之采用免疫荧光双标记二维流式细胞仪分析法检测三个ＰＣＤ相关调控蛋白在ＣＤ３４＋造血细胞上的表达。结果流式细胞仪及免疫桥酶联组化鉴定ＣＤ３４＋造血细胞的纯度达９０％～９５％。Ｐ５３在ＣＤ３４＋造血细胞上的表达为０．２％～０．８％、ｃ－ｍｙｃ为１．９％～１１．３％、ｂｃｌ－２为０．４％～１．５％；而在骨髓单个核细胞则分为０．１％～０．６％、０．１％～０．４％和０．１％～０．８％。结论正常生理状况下，造血细胞的生存、增殖、分化、成熟及死亡与Ｐ５３、ｃ－ｍｙｃ及ｂｃｌ－２调控蛋白具有密切相关性。
bjective To show the coexpression levels of P53,c myc or bcl 2,three key apoptosis regulatingproteins on CD34 +HC and bone marrow mononuclear cells(BMMNC).Methods Isolex TM 50 or CIMS 100 systems of immunomagnetic bead separation and two dimensional flow cytometric analysis of the immunofluorescence double staining were used.Results With90% 95%purity of CD34 +HC measured by FACS,we found that expression levels of P 53,c myc,and bcl 2 on both CD34 +HC and BMMNC were as followings:CD34 +/P53+0.1 0.8% versus BMMNC 0.1 0.6%;CD34 +/c myc +2.4%～11.3% versusBMMNC 0.1 0.4%;CD34 +/bcl 2 +0.5 1.6% versus BMMNC 0.1 0.8%.Coexpression of c myc and bcl 2 on CD34 +HC was significantly higher than those observed on BMMNC respectively.Conclusion It could be speculated that apoptosis regulating proteins P53,c myc and bcl 2play akey role in the control of the survival,proliferation,differ entiation,maturation and death in hematopoietic cells of physiological state,suggesting that these proteins balance the production numbers and quility of hematopoietic cells.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第3期197-200,共4页
National Medical Journal of China
基金
国家自然科学基金
