摘要
应用免疫组化ＡＢＣ法研究了金黄地鼠视网膜酪氨酸羟化酶免疫反应（ＴＨ－ⅠＲ）神经元的定位和分布。结果表明８４．６％的ＴＨ－ⅠＲ神经元是无长突细胞或网间细胞；１５．４％是移位无长突细胞或视网膜节细胞（ＲＧＣ）。ＴＨ－ⅠＲ细胞突起主要分布在内网层（ＩＰＬ）的第１亚层。ＴＨ－ⅠＲ神经元分布于视网膜中央区和周围区，其密度为（２２．４±１．５）个／ｍｍ２。颞上区密度最高，为（２９．１±３．２）个／ｍｍ２。ＴＨ－ⅠＲ无长突细胞多为星形。在节细胞层（ＧＣＬ）的ＴＨ－ⅠＲ细胞均为中小型神经元，胞体直径约为７～１６μｍ，它们的突起分支在ＩＰＬ的第１亚层。荧光素逆行标记结合间接免疫荧光组化的双标研究，表明金黄地鼠视网膜的ＧＣＬ内，部分ＴＨ－ⅠＲ神经元是ＲＧＣ。ＴＨ－ⅠＲＲＧＣ数目约为（５５±７）个，约占ＧＣＬ的ＴＨ－ⅠＲ神经元的４５％。
The localization and distribution of TH ⅠR neurons in hamster retina was studied with immunohistochemistry ABC method. The results showed that 84.6% TH ⅠR cells were amacrine cells (AC) or interplexiform cells(IPC). 15.4% TH ⅠR neurons were displaced amacrine cells and/or ganglion cells (RGC). The processes of TH ⅠR cells ramified most heavily in sublaminal 1 of innerplexiform layer(IPL). TH ⅠR cells were distributed throughout the central and periphery of the retina. The density of TH ⅠR cells was calculated to be 22.4±1.5 cells per mm 2. The hightest density (29.1±3.2 cell/mm 2) of TH ⅠR cells occured in superior temporal retina. The majority of TH ⅠR AC exhibited a satellite appearance. The TH ⅠR cells in GCL were medium to small sized. The diameter of these neurons ranged from 7￣16 μm. And their processes ascended to sublamina 1 of IPL. The double label study of fluoresence retrograde tracing combined with TH indirect immunofluoresence histochemical method showed that some TH ⅠR neurons in the GCL of hamster retina were RGC. The number of TH ⅠR RGC was estimated to be 55±7, representing 45% of the total number of TH ⅠR neurons in GCL.
出处
《中山医科大学学报》
CSCD
1997年第1期19-23,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金
