摘要
本文用ＥＬＩＳＡ间接法检测了一种恶性疟原虫保护性抗原重组复合基因表达产物诱导小鼠体液免疫的反应。该复合基因包含了编码ＭＳＡ１、ＭＳＡ２和ＲＥＳＡ上的Ｔ和／或Ｂ细胞刺激位点的基因片段，同时加有ＩＬ－１和ＴＴ上的广谱Ｔ细胞刺激位点的基因片段。该复合基因在Ｅ．ｃｏｌｉ中的表达产物（简称复合抗原）在有和无佐剂的条件下免疫ＢＡＬＢ／ｃ小鼠后，均产生了明显的抗复合抗原的抗体，同时也产生了明显的抗可溶性裂殖子抗原的抗体。相反，用可溶性裂殖子抗原免疫的ＢＡＬＢ／ｃ小鼠则不能产生明显的抗复合抗原的抗体。另外。
Li quan zhen et al. constructed a hybrid gene(HG)which codes for several T/B cell epitopes of MSA1/1, MSA2/2, RESA and universal T cell epitopes of IL 1, tetanus toxoid. The HG was cloned into expression vector pWR450 1, and expressed in E.coli to produce a fusion protein(HRA)(fused to β galactosidase) with molecular weight of 65 kD. The humoral immunity in BALB/c and C57BL/6 mice induced by HRA and soluble merozoite antigen(SMA) were investigated. BALB/c mice produced prominent anti HRA antibody after immunized with HRA emulsified with Freund′s adjuvant or HRA alone. They could also produce antibody to SMA significantly. Inversly, BALB/c mice injected with SMA could not raise antibody to HRA. C57BL/6 mice could also raise antibody to HRA significantly after immunized with HRA. These results suggest that HRA is immunogenic and may overcome MHC restriction.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1997年第1期16-19,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
联合国开发计划署/世界银行/世界卫生组织热带病研究和培训特别规划署(TDR)资助
