摘要
探讨一种通过以超临界萃取脱脂和化学超声浸泡脱蛋白为主处理天然骨的新方法,制备一系列天然骨材料,并对其进行了扫描电子显微镜(SEM)、X射线衍射(XRD)及热失重(TGA)表征。研究结果表明,处理后骨材料的性能与样品的体积、处理步骤的顺序及处理时间有密切的关系,得到的天然骨保持了多孔结构,孔壁干净光滑,未见其它残留物质,骨中羟基磷灰石晶体结构保持良好,且有机成分大大减少。更重要的是本研究的处理方法与传统的处理方法相比较,不仅能较彻底地脱脂,而且还避免了有机溶剂的使用,是一种新型的异种骨处理方法。
A new method was used for treating the natural bone. A series of natural bone materials were treated mainly by Supercritical Carbon Dioxide Fluid Technology （SC-CO2-FT） and chemical ultrasound marination. The treated bone materials were determined by scanning electron microscopy, X-ray diffraction and thermo gravimetric analyses. The results showed that the sizes of bone, the sequence of processes as well as the time effected the treated bone. The treated bone material still maintained its natural porous structure, and its wall was quite clear; moreover, the hydroxyapatite crystals kept good crystalline structure. Compared to traditional treatments, the new treatments were beneficial to delipidation and proteic extraction and were not in need of organic solvent. The results demonstrated that this new method is promising in the treatment of natural bone.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2007年第2期332-335,共4页
Journal of Biomedical Engineering
基金
国家"973"资助项目(G1999054306)
"863"资助项目(2001AA625050)
广东省"十五"重大专项资助项目(A302020104)
关键词
超临界二氧化碳
骨脱脂
脱蛋白
Supercritical carbon dioxide Bone delipidation Deprotein
