摘要
目的:获取高纯度蒜氨酸并研究其部分功能。方法:采用改进的方法提取蒜氨酸。用体外实验检测其抗菌和抗肿瘤活性。结果:获得了高纯度的蒜氨酸,抑菌实验表明,高纯蒜氨酸对大肠杆菌(Eschrichia coli)、枯草芽孢杆菌(Bacillus subtilis)、金黄色葡萄球菌(Staphylococcus aureus)、痢疾杆菌(Shigella dysenteriae)、幽门螺旋杆菌(Helicobacter pylori)等测试菌株有极强的杀死作用,其MIC分别为4.06×10-3mg/mL、4.06×10-3mg/mL、4.06×10-3mg/mL、8.13×10-3mg/mL、1.63×10-2mg/mL,体外细胞实验结果显示,蒜氨酸对肿瘤细胞人急性早幼粒白血病细胞(HL-60)和人肝癌细胞(H-7402)有很高的抑制和杀死作用。结论:该提取方法适合于大规模生产,蒜氨酸在食品防腐、抗肿瘤药物开发和保健品的研制上有很好的应用潜力和应用前景。
Objective： To obtain highly purified alliin and study part of its function.Methods：An improved purification method was used in this experiment.The antibacterial and antitumor activity of alliin were tested in vitro.Results： Alliin was obtained by the simple method.And it can inhibit the growth of Eschrichia coli,Bacillus subtilis,Staphylococcus aureus,Shigella dysenteriae and other tested strains,the MIC were 4.06×10^-3mg/mL,4.06×10^-3mg/mL,4.06×10^-3mg/mL,8.13×10^-3mg/mL,1.63×10^-2mg/mL separately.Conclusion：The method was fit for industry production,On the other hand,alliin have great effects on tumor cells such as HL-60 and H-7402.It demonstrated that alliin could be used for health care,food preservative,medicine and so on .
出处
《生物技术》
CAS
CSCD
2007年第2期59-61,共3页
Biotechnology
关键词
大蒜
蒜氨酸
提取
生理活性
garlic
alliin
extraction
physiological activity
