摘要
目的探讨胆汁淤积型肝病碱性磷酸酶活性的检测方法,为胆汁淤积型肝病的临床诊断提供可靠的生化指标和检测方法。方法利用结扎胆总管方法建立胆汁淤积型肝病大鼠模型,分别用二乙醇胺、2-乙基氨基乙醇、2-氨基-2-甲基-1-丙醇3种不同缓冲液的试剂方法,测定大鼠血清碱性磷酸酶的活性,并作比较分析。结果实验组血清碱性磷酸酶的活性随着胆汁淤积时间增长而升高,胆总管结扎5d后血清碱性磷酸酶活力与对照组相比,差异有显著(P<0.01);使用2-乙基氨基乙醇缓冲液的试剂方法,测定血清碱性磷酸酶的活性值最高,与其他两种方法的测定结果比较分析,差异均有显著性(P<0.01)。结论血清碱性磷酸酶是胆汁淤积型肝病临床诊断的重要生化指标;用2-乙基氨基乙醇缓冲液的试剂方法,测定血清胆汁淤积型肝病碱性磷酸酶活性,效果较为理想。
[Objective] To study the detecting method of the alkaline phosphatase activity in order to offer a credible biochemical indicator and detecting method in liver disease of cholestasis. [Method] TO establish a rat model of cholestasis by ligating bile commen duct. The alkaline phosphatase activities were determined by using the different buffer solutions such as diethanolamine, 2-ethyl aminoethanol and 2-amino-2-methyl-1-propanol and the results were compared. [Result] The serum alkaline phosphatase activities went up along with the ligating time in experiment group and marked difference were be seen as compare to controls after five days of ligation （P 〈0.01）. The alkaline phosphatase activities were highest by using the method of 2-ethyl aminoethanol buffer solution compared with the results of the other two kinds of methods （P 〈0.01）. [Conclusion] The serum alkaline phosphatase is an important biochemical index in clinic diagnosis of cholestasis. The method of using 2-ethyl aminoethanol buffer solution to determine alkaline phosphatase activity is more ideal.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第7期800-802,共3页
China Journal of Modern Medicine
