摘要
目的探讨梗阻性黄疸围手术期肝脏增殖细胞核抗原及细胞间粘附因子1表达,以及应用生长激素对肝脏增殖细胞核抗原及细胞间粘附因子1表达的影响。方法采用梗阻性黄疸动物模型,胆总管双重结扎法建立胆道梗阻。将45只大鼠随机均分为假手术组(SO)、梗黄组(OJ)及生长激素组(rhGH)。OJ组、rhGH组行胆道结扎、切断,而SO组不结扎胆道,等待两周模型建立。rhGH组每天皮下注射Saizen0.75u/kg,SO组及OJ组相同计量NS对照,共两周。检测肝脏形态学改变,同时检测血胆红素、内毒素及PCNA及ICAM-1的肝内表达。结果梗阻性黄疸组血清内毒素含量为(0.77±0.03)u。血清总胆红素、直接胆红素、白蛋白水平和合丙较氨酶分别为(186.64±49.59)μmol/L,(144.64±41.09)μmol/L,(18.77±2.01)g/L,(180.42±30.88)u/L。而在生长激素组以上数据分别为(150.51±24.28)μmol/L,(104.98±16.10)μmol/L,(23.43±2.76)g/L,and(125.69±29.61)u/L。免疫组织化学法检测肝脏ICAM-1和PCNA的表达显示生长激素组较梗阻性黄疸组差异显著,具有统计学意义。结论内毒素会导致梗阻性黄疸围手术期肝细胞损伤及肝功能异常,而生长激素可有效缓解这一损伤。
[Objective] To investigate proliferative cell nuclear antigen and intercellular adhesive molecular-1 expression in rat liver cells suffered from common bile duct ligation, whether somatotrophin has modulating effect on promoting hepatocyte immune modulation. [Methods] Obstructive jaundice model was used in this experiment. In the model rat＂ s common bile duct was double-ligated. Serum endotoxin, bilirubin, and albumin were measured with HITACHI automatic biochemistry analyzer. Hepatic ICAM-1 and PCNA analysis were detected and analyzed to compare with immune injury. [Results] Serum endotoxin level in OJ group was （0.77±0.03） u. The values of T-BIL, D-BIL,ALB and GPT in OJ group were （186.64±49.59） μmol/L, （144.64±41.09） μmol/L, （18.77±2.01） g/L and （180.42±30.88） u/L. In somatotrophin group they were （150.51± 24.28） μmol/L, （104.98±16.10）μmol/L, （23.43±2.76） g/L and （125.69±29.61） u/L. ICAM-1 and PCNA expression analysis in two groups showed what in somatotrophin group was better. [Conclusions] In obstructive jaundice endotoxin could hurt hepatocyte and hepatic function. On the other side somatotrophin could recover hepatocyte function and eliminate hepatic injury.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第7期773-776,781,共5页
China Journal of Modern Medicine
关键词
生长激素
免疫调节
梗阻性黄疸
细胞间粘附分子-1
增生细胞核抗原
somatotrophin
immune modulation
obstructive jaundice
intercellular adhesive molecular-1
proliferative cellular nuclear antigen
