摘要
目的对中药辛夷的抗炎效应初步探讨。方法利用TNF—α诱导活化的内皮细胞与中性粒细胞的体外黏附实验与肾缺血再灌注损伤大鼠模型，观察辛夷挥发油对中性粒细胞黏附功能的影响以及肾损伤的保护作用。结果体外培养的人脐静脉内皮细胞在TNF—α刺激后，与中性粒细胞发生黏附，黏附量为248．6±．13．8；而辛夷挥发油处理后，黏附量明显降低，在0．5、1．0,2．0ul/ml浓度的黏附量分另4为191.6±8．2，158．2±9.0，155．2±9．7（均P〈0．05）。缺血再灌注后，肾组织出现明显炎症损伤改变．BUN、Ser水平明显升高，但辛夷治疗组肾脏组织病理改变与肾功能损害较轻。结论本实验条件下，辛夷具有一定抗炎效应。
Objective To explore the anti-inflammation effects of magnoliae fargesii volatile oil. Methods Stimulating the human umbilical vein endothelial cells （HUVECs） to express cell adhesion molecules with TNF-α, then the anti-adhesion effects of magnoliae fargesii volatile oil between HUVECs and human peripheral neutrophils were observed. Using a rat model of 60 minutes renal ischemia followed by 1,3, 6 and 24 hours reperfusion, animals were randomly divided into following groups ： the sham-operated controls, ischemic group receiving only normal saline, and treated group receiving magnoliae fargesii volatile oil before reperfusion. Then the renal injuries of rats were observed. Results The cell adhesion between HUVECs and neutrophils was high （248. 6 ± 13. 8）. Magnoliae fargesii volatile oil could block the adhesion process at the concentration of 0. 5 ul/ml （ 191.6 ± 8.2 ）, 1. 0ul/ml （ 158.2 ±9, 0 ） ,2.0ul/ml （ 155.2 ± 9. 7 ）, （ either P 〈 0. 05）. The anti-adhesion effects would be strengthened with the increase of volatile oil concentration. BUN and SCr levels of the rats were significantly increased after 24 hours reperfusion while the increment was remaxkably attenuated by treatment with magnoliae fargesii volatile oil. The renal injuries were severe after 1 hour reperfusion, which was remarkably attenuated by treatment with magnoliae fargesii volatile oil. Conclusions The results suggest that magnoliae fargesii volatile oil have anti-inflammation effects.
出处
《中国医师杂志》
CAS
2007年第4期455-457,共3页
Journal of Chinese Physician
基金
上海市卫生局基金项目（004Y07）,院级课题项目（0239）
