摘要
目的建立垂体腺瘤动物模型,比较常用雌激素药物苯甲酸雌二醇与己烯雌酚诱导Fischer344大鼠泌乳素腺瘤的差异。方法采用F344雌性大鼠,随机分成腹腔注射己烯雌酚实验组和苯甲酸雌二醇实验组。用药50d后行MRI扫描,观察大鼠生存状态,检测垂体重量、血清催乳激素（PRL）水平、病理与PRL免疫组织化学改变。结果用药后两组大鼠体重增长均明显受抑,苯甲酸雌二醇实验组垂体重量与PRL水平显著升高,MRI可见增大垂体,病理可见垂体肿瘤形成且PRL染色阳性,成瘤率为100%;己烯雌酚实验组未见垂体腺瘤形成。结论苯甲酸雌二醇可短期稳定诱导F344大鼠形成垂体腺瘤,且符合泌乳素腺瘤的生物学特性,是建立垂体腺瘤动物模型的理想方法。
Objective To induce prolactinoma formation in Fischer344 rats and compare the efficiency of estradiol benzoate and diethylstilbestrol Methods Female Fischer344 rats were divided into estradiol benzoate-treated group and diethylstilbestrol - treated group. After 50 days of treatment, we observed the rat living status and evaluate the prolactinoma formation by MRI scan, pituitary weight, serum prolactin level, HE stain and PRL immunohistochemistry stain results. Results The growth of rat body weight in both group were retarded obverously after the use of estrogen. All estradiol benzoate-treated rots formed the prolactinoma after 50 days of treatment. Pituitary weight and serum PRL increased significantly in comparison with the normal rats. Pituitary adenomas were found in MRI scan and HE stain sections and they were PRL stain positive. But diethylstilbestrol - treated rats didn ＇t form prolactinoma at the same time Conclusions Estradiol benzoate can induce the formation of prolactinoma in Fischer344 rats. The method is easy with short tumor formation period and stable tumor accidence, It＇s the idealized animal model in pituitary adenoma research.
出处
《中华神经外科杂志》
CSCD
北大核心
2007年第3期217-219,共3页
Chinese Journal of Neurosurgery
