摘要
目的:探讨肺癌患者血清中癌抗原识别分子及其抗肿瘤机制。方法:免疫亲合层析法分离纯化肺癌患者外周血中肺癌膜蛋白识别分子,SDS-PAGE电泳法分析其相对分子质量。MTT法测定细胞增殖情况;Annexin V/PI双标法检测细胞凋亡率,并通过流式细胞仪检测p53、Bcl-2蛋白表达水平。结果:肺癌患者外周血中存在肺癌膜蛋白识别分子,相对分子质量分别约为70×103、66×103和15.2×103。不同浓度膜蛋白识别分子(10、20和40mg/L)具有抑制肺癌细胞株(GLC-82)生长和促进细胞凋亡作用。肺癌细胞株与肺癌膜蛋白识别分子共培养6h后,Bcl-2蛋白表达水平明显降低,P=0.008;而p53水平明显增加,P=0.000。结论:肺癌患者外周血中存在肺癌膜蛋白识别分子,并具有抑制肺癌细胞增殖和促进凋亡作用,其机制与p53和Bcl-2表达改变有关。
OBJECTIVE： To study the mechanism of molecules recognizing tumor-antigen against cancer in the serum of lung cancer patients. METHODS： The molecules, which could recognize mem brane proteins of lung cancer tissues, were separated by immune affinity chromatography, and then purified and analyzed by SDSPAGE. The proliferation changes in GLC-82 cells were analyzed by MTT chronometry. ＂[＇he flow cytometry was used to detect apoptosis, and analyze the changes of expressions of p53 and Bcl-2 proteins in GLC-82 cells. RESULTS： The molecules, which could recognize membrane proteins of lung cancer tissues, were separated and purified with the molecular weight of 70 000, 66 000 and 15 20（1. Those markedly inhibited cell proliferation and viability, and prompted apoptosis in GLC 82 cells at 10, 20 and 40 mg/L. The mean fluorescent intensity of expression of Bel-2 protein （P = 0. 008） in GLC-82 cells significantly decreased, however, of p53 proteins significantly increased at 6 h after co-cultured with drug decreased, P=0. 000. CONCLUSIONS： There are molecules, recognizing the membrane proteins of lung cancer tissues in the sera of lung cancer patients, which could inhibit GLC 82 cells proliferation and induce apoptosis. Its mechanism is related to the changes of expressions of p53 and Bcl-2.
出处
《中华肿瘤防治杂志》
CAS
2007年第5期332-334,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
广东省科学基金(A2003615)
关键词
肺肿瘤
细胞凋亡
膜蛋白质类
lung neoplasms
apoptosis
membrane proteins
