摘要
目的:研究酸枣仁汤对小鼠试验性急性肝衰竭的影响。方法:雄性昆明小鼠,给予D-半乳糖胺(D-Gal-N)和脂多糖(LPS)同时腹腔注射,制备小鼠急性肝衰竭模型;并于造模前2 h,治疗组分别给予相应药物灌胃,正常、模型组给予蒸馏水灌胃。比较各组小鼠的24 h存活率,血清转氨酶水平,肝脏组织病理学改变,血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)的水平,肝脏组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽还原酶(GR)、一氧化氮(NO)、一氧化氮合酶[NOS包括总一氧化氮(T-NOS)和诱生型一氧化氮(iNOS)]的水平。结果:D-Gal-N/LPS可以导致小鼠急性肝衰竭;酸枣仁汤可以提高小鼠存活率,减轻肝脏病变程度,降低血清转氨酶活性及TNF-,αIL-1β的浓度,增加肝脏组织中SOD,GR的活性,降低NOS的活性及MDA,NO的浓度。结论:酸枣仁汤可减轻D-Gal-N/LPS所致的急性肝衰竭,提高小鼠的存活率,其作用机制可能与它影响睡眠从而影响炎性细胞因子(如TNF-,αIL-1)的释放和机体氧化能力的改变有关。
Objective： To investigate the effect of Suanzao nacute hepatic failure in mice. Method： Acute liver failure was induced in male Kunming strain mice by enterococlia injecting the animals with D-Gal-N and LPS. The mice in treatment groups were given corresponding drug 2 h before administration of D-Gal-N and LPS, and the mice in control group were given the same dose of distilled water. The 24 h survival rate, serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST） levels were compared. Serum the levels of TNF-α and IL-1 and the levels of SOD, MDA, GR, GSH, NO and NOS in the liver were determined. Result. Treatment with Suanzaoren decoction could increase the survival rate and improve the liver histological feather. Suanzaoren decoction inhibited the serum the levels of ALT, AST, TNF-α and IL-1, and reduced the levels of MDA, NO and NOS and increased the levels of GR and SOD in the liver. Conclusion： Treatment with Suanzaoren decoction can suppress the D-Gal-N/LPS-induced acute hepatic failure. It may be the mechanism that Suanzaoren decocotion regulate the production of inflammatory cytokines and free radicals.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第8期718-721,共4页
China Journal of Chinese Materia Medica
关键词
酸枣仁汤
肝衰竭
睡眠
Suanzaoren decoction
hepatic failure
sleep
