摘要
利用15^N标记研究了不同基因型玉米氮素利用效率的差别；分析了不同基因型玉米豫单2002（高蛋白）和豫单136（低蛋白）籽粒中蛋白质含量及单粒蛋白质含量在灌浆期的动态变化；测定了籽粒中硝酸还原酶和谷氨酰胺合成酶活性的变化动态，结果表明蛋白质含量高的．玉米品种氮素利用率较高，豫单2002比豫单136高7．79％；二者的硝酸还原酶活性在灌浆期内变化趋势相近，授粉0～30d，也相差不大，授粉后30d至收获，豫单2002的硝酸还原酶活性始终高于豫单136，授粉后第45天时，豫单2002比豫单136高出32．77μgNO2^-／（gFW·h）；豫单2002与豫单136籽粒的谷氨酰胺合成酶活性在籽粒灌浆期的变化动态均呈“∽”型，且豫单2002一直高于豫单136；高的酶活性促进了蛋白质的形成。本研究为高蛋白玉米的蛋白质形成基础提供了理论依据。
The difference of nitrogen utilization efficiency of different genotype maize was studied by 15 N labeled fertilizer. The changes of protein content, nitrate reductase （NRase） and glutamine synthetase （GS） in the grain and protein quality in single grain were analyzed. The results showed that nitrogen utilization efficiency of high protein maize variety（Yudan2002） was 7. 79% higher than that of low protein maize variety（Yudan136） ;The change tendency of NRase in both varietes was similar. NRase activity in Yudan2002 was higher than that in Yudan136 from the 30^th day to the 452 day after pollination NRase activity in Yudan2002 was 32.77μg NO2^-（g FW·h） higher than that in Yudan136 on the 45^th day after pollination. The pattern of GS activity was the same as ＂∽＂ in both Yudan2002 and Yudan136 during the fillingstage, but GS activity in Yudan2002 was higher than that in Yudan136. High activity is benefit to protein formation. This paper provides more theoretical evidence to the mechanis of high protein production of maize.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第2期173-176,共4页
Journal of Nuclear Agricultural Sciences
