摘要
目的建立了测定中药材人参中多菌灵农药残留量方法。方法采用RP-HPLC法，Diamonsil C18色谱柱。流动相为甲醇-四氢呋喃-水（43：2：55），检测波长为277nm。结果平均回收率人参茎叶83．94％，RSD为2．42％（n=6）；平均回收率人参根87．38％，RSD为2．68％（n=6）。多菌灵在1．926～192．6ng时，r=1．0000，呈良好的线性关系。结论本方法简便、快速、适合同类中药材中多菌灵农药残留量测定与安全监控。
OBJECTIVE To establish a HPLC method for the determination of residual carbendazim in Chinese Traditional Medicine of ginseng. METHODS The mobile phase constituted of methanol-tetrahydrofuran-water （ 45 ： 2：55 ） . A Diamonsil C18 column was used, and carbendazim was determined at the detection wavelength of 277 nm. RESULTS The averrage recovery and RSD of ginseng stems and leaves were 83.94% and 2. 42% （n = 6）, respectively. The averrage recovery and RSD of ginseng radix were 87.38% and 2. 68% （n = 6） ,respectively, The standard curve of carbendazim was linear in the range of 1. 926 - 192. 6 ng（r = 1. 000 0）. CONCLUSION This method is simple, rapid and reliable. It is suitable for the determination and safe control of residual carbendazim in this kind of Chinese Traditional Medicine.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第6期475-477,共3页
Chinese Pharmaceutical Journal
基金
国家科技部科研院所社会公益项目(2004DAB17039)
