摘要
目的研究供应结肠癌组织的动脉对缩血管物质的反应性。方法取结肠癌患者供应癌组织的动脉(癌动脉),以供应癌周正常组织的动脉(癌周动脉)和交通事故死亡者对应部位的动脉(正常动脉)为对照,动脉切成长约1mm的动脉环,记录4种缩血管物质对动脉收缩的量效曲线。结果去甲肾上腺素(NA)引起正常动脉的最大收缩(Emax)和半最大效应浓度(pEC50)分别为97%±19%和5.94±0.17(n=5);NA收缩癌动脉的Emax和pEC50分别为74%±5%和5.54±0.21(n=5),NA收缩癌周动脉的Emax和pEC50分别为119%±11%和5.84±0.09(n=5)。NA收缩癌动脉的Emax较癌周动脉及正常动脉的Emax值降低(P<0.01,P<0.05)。内皮素-1(ET-1)强烈收缩癌周动脉,其Emax和pEC50分别为248%±34%和8.37±0.04。与癌周动脉的量效曲线比较,ET-1收缩正常动脉和癌动脉的量效曲线明显右移、压低。5-HT和S6c对正常动脉、癌动脉及癌周动脉无明显的缩血管作用。结论ET-1和NA是收缩结肠癌动脉的主要缩血管物质,癌动脉对ET-1和NA收缩血管的反应性明显低于癌周动脉和正常动脉。
Aim The present study was designed to investigate the contractile responses to vasoconstrictors in artery supplying colon carcinoma. Methods The artery supplying carcinoma was obtained from patients suffering from colonic carcinoma. The artery supplying carcinoma ambient normal tissue from patients with colon carcinoma and normal artery from patients with accident were used to control. Artery were cut into about 1 mm long cylindrical segments. The concentrationcontractile curves induced by 4 vasoconstrictors were recorded. Results In the normal artery, the Emax and pEC50 of contraction induced by noradrenaline （NA） were 97% ± 19% and 5.94 ±0. 17 （ n = 5 ） , respectively; while in the artery supplying carcinoma, the Emax and pEC50 of contraction induced by NA were 74%±5% and 5.54 ±0.21（n =5）. In the artery supplying cancer ambient normal tissue, the Emax and pEC50 of contraction induced by NA were 119% ± 11% and 5.84 ± 0. 09, respectively. The Emax in artery supplying carcinoma induced by NA was lower than that in normal arteries （ P 〈 0. 05 ） and that in the artery supplying cancer ambient tissue （ P 〈 0. 01 ）. Endothelin-1 （ ET-1 ） markedly contracted the artery supplying cancer ambient normal tissue with the Emax of 248%±34% and the pEC50 of 8.37 ± 0. 04 （ n = 5 ）. Compared to concentration -contractile curve of the artery supplying cancer ambient normal tissue, the concentration-contractile curves of the artery of the normal artery and the artery supplying carcinoma shifted toward right and depressed. 5-hydroxytryptamine and sarafotoxin 6c did not induce obvious contraction on the normal artery, the artery supplying cancer ambient normal tissue and the artery supplying colon carcinoma. Conclusion ET-1 and NA are main vasoconstrictors contracting the artery supplying colonic carcinoma. The contractile responses of the artery supplying carcinoma to ET-1 and NA are much less than that in the artery supplying cancer ambient normal tissue and the normal artery.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2007年第4期446-450,共5页
Chinese Pharmacological Bulletin
基金
教育部高等学校科技创新工程重大项目培育基金(No705045)
