摘要
目的构建博尔纳病病毒p40基因重组表达质粒。方法通过PCR方法扩增获得博尔纳病病毒p40基因的完整序列,将此片段定向克隆到pEGFP-N1载体多克隆位点区,筛选重组阳性菌株,提取重组质粒,利用PCR方法和核酸序列测定验证重组质粒构建的正确性。结果成功构建博尔纳病病毒p40基因重组表达质粒。结论本文构建的重组质粒将为研究博尔纳病病毒p40基因在真核细胞中的功能和作用提供实验依据。
Objective To construct the plasmid expressing BDV p40 gene. Methods The target DNA fragments of BDV p40 gene was obtained by PCR amplification and inserted into pEGFP-N1 reporter vector. The constructed recombinant plasmid was confirmed by PCR and DNA sequencing. Results The recombinant plasmid pEGFP-BDVp40 was constructed and identified by DNA sequencing as same as the template of p40 gene of BDV H1766 strain. Conclusion The successfully constructed pEGFP-BDVp40 plasmid is useful to study the gene＇s function and effects in eukaryotic cells.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2007年第2期102-104,共3页
Journal of Harbin Medical University
基金
国家自然科学基金(30371242)
黑龙江省杰出青年科学基金(JC04-05)
教育部高校博士点专项基金(20040226011)
黑龙江省教育厅骨干教师创新能力项目
